Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo

Conrad William Merkle, Shau Poh Chong, Aaron Michael Kho, Jun Zhu, Alfredo Dubra, Vivek Srinivasan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Most flying-spot optical coherence tomography and optical coherence microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single-mode optical fiber. Here we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a water immersion objective on the illumination path while maintaining a conventional Gaussian mode detection path (1∕e2 intensity diameter ~0.82 Airy disks), enabling ~1.1 μm full width at half-maximum (FWHM) transverse resolution. At the same time, a ~0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory and, finally, used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull.

Original languageEnglish (US)
Pages (from-to)198-201
Number of pages4
JournalOptics Letters
Volume43
Issue number2
DOIs
StatePublished - Jan 15 2018

Fingerprint

brain
microscopy
illumination
water immersion
axons
skull
erythrocytes
mice
light sources
tomography
optical fibers
flight
broadband
symmetry
geometry
scattering

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo. / Merkle, Conrad William; Chong, Shau Poh; Kho, Aaron Michael; Zhu, Jun; Dubra, Alfredo; Srinivasan, Vivek.

In: Optics Letters, Vol. 43, No. 2, 15.01.2018, p. 198-201.

Research output: Contribution to journalArticle

Merkle, Conrad William ; Chong, Shau Poh ; Kho, Aaron Michael ; Zhu, Jun ; Dubra, Alfredo ; Srinivasan, Vivek. / Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo. In: Optics Letters. 2018 ; Vol. 43, No. 2. pp. 198-201.
@article{24efb425547c4d8f8124b3def40b524f,
title = "Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo",
abstract = "Most flying-spot optical coherence tomography and optical coherence microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single-mode optical fiber. Here we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a water immersion objective on the illumination path while maintaining a conventional Gaussian mode detection path (1∕e2 intensity diameter ~0.82 Airy disks), enabling ~1.1 μm full width at half-maximum (FWHM) transverse resolution. At the same time, a ~0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory and, finally, used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull.",
author = "Merkle, {Conrad William} and Chong, {Shau Poh} and Kho, {Aaron Michael} and Jun Zhu and Alfredo Dubra and Vivek Srinivasan",
year = "2018",
month = "1",
day = "15",
doi = "10.1364/OL.43.000198",
language = "English (US)",
volume = "43",
pages = "198--201",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "2",

}

TY - JOUR

T1 - Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo

AU - Merkle, Conrad William

AU - Chong, Shau Poh

AU - Kho, Aaron Michael

AU - Zhu, Jun

AU - Dubra, Alfredo

AU - Srinivasan, Vivek

PY - 2018/1/15

Y1 - 2018/1/15

N2 - Most flying-spot optical coherence tomography and optical coherence microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single-mode optical fiber. Here we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a water immersion objective on the illumination path while maintaining a conventional Gaussian mode detection path (1∕e2 intensity diameter ~0.82 Airy disks), enabling ~1.1 μm full width at half-maximum (FWHM) transverse resolution. At the same time, a ~0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory and, finally, used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull.

AB - Most flying-spot optical coherence tomography and optical coherence microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single-mode optical fiber. Here we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a water immersion objective on the illumination path while maintaining a conventional Gaussian mode detection path (1∕e2 intensity diameter ~0.82 Airy disks), enabling ~1.1 μm full width at half-maximum (FWHM) transverse resolution. At the same time, a ~0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory and, finally, used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull.

UR - http://www.scopus.com/inward/record.url?scp=85040308435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040308435&partnerID=8YFLogxK

U2 - 10.1364/OL.43.000198

DO - 10.1364/OL.43.000198

M3 - Article

VL - 43

SP - 198

EP - 201

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 2

ER -