Abstract
Most flying-spot Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single mode optical fiber. Here, we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a 0.3 numerical aperture (NA) water immersion objective on the illumination path, while maintaining a conventional Gaussian mode detection path (1/e2 intensity diameter ∼0.82 Airy disks), enabling ∼1.1 μm full-width at half-maximum (FWHM) transverse resolution. At the same time, a ∼0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory, and then used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull. Finally, by introducing a 0.8 NA water immersion objective, we improved the lateral resolution to 0.44 μm FWHM, which provided a volumetric resolution of ∼0.2 fL, revealing cell bodies in cortical layer I of the mouse brain with OCM for the first time.
| Original language | English (US) |
|---|---|
| Title of host publication | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII |
| Editors | Valery V. Tuchin, Valery V. Tuchin, James G. Fujimoto, Joseph A. Izatt |
| Publisher | SPIE |
| Volume | 10483 |
| ISBN (Electronic) | 9781510614512 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Event | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII 2018 - San Francisco, United States Duration: Jan 29 2018 → Jan 31 2018 |
Other
| Other | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII 2018 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 1/29/18 → 1/31/18 |
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Keywords
- cell bodies
- meninges
- mouse cortex
- myelin
- Optical coherence microscopy
- optical coherence tomography
- red blood cell
- visible light
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
Visible light optical coherence microscopy imaging of the mouse cortex with femtoliter volume resolution. / Merkle, Conrad W.; Chong, Shau Poh; Kho, Aaron M.; Zhu, Jun; Kholiqov, Oybek; Dubra, Alfredo; Srinivasan, Vivek.
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII. ed. / Valery V. Tuchin; Valery V. Tuchin; James G. Fujimoto; Joseph A. Izatt. Vol. 10483 SPIE, 2018. 104831X.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Visible light optical coherence microscopy imaging of the mouse cortex with femtoliter volume resolution
AU - Merkle, Conrad W.
AU - Chong, Shau Poh
AU - Kho, Aaron M.
AU - Zhu, Jun
AU - Kholiqov, Oybek
AU - Dubra, Alfredo
AU - Srinivasan, Vivek
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Most flying-spot Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single mode optical fiber. Here, we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a 0.3 numerical aperture (NA) water immersion objective on the illumination path, while maintaining a conventional Gaussian mode detection path (1/e2 intensity diameter ∼0.82 Airy disks), enabling ∼1.1 μm full-width at half-maximum (FWHM) transverse resolution. At the same time, a ∼0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory, and then used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull. Finally, by introducing a 0.8 NA water immersion objective, we improved the lateral resolution to 0.44 μm FWHM, which provided a volumetric resolution of ∼0.2 fL, revealing cell bodies in cortical layer I of the mouse brain with OCM for the first time.
AB - Most flying-spot Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single mode optical fiber. Here, we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a 0.3 numerical aperture (NA) water immersion objective on the illumination path, while maintaining a conventional Gaussian mode detection path (1/e2 intensity diameter ∼0.82 Airy disks), enabling ∼1.1 μm full-width at half-maximum (FWHM) transverse resolution. At the same time, a ∼0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory, and then used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull. Finally, by introducing a 0.8 NA water immersion objective, we improved the lateral resolution to 0.44 μm FWHM, which provided a volumetric resolution of ∼0.2 fL, revealing cell bodies in cortical layer I of the mouse brain with OCM for the first time.
KW - cell bodies
KW - meninges
KW - mouse cortex
KW - myelin
KW - Optical coherence microscopy
KW - optical coherence tomography
KW - red blood cell
KW - visible light
UR - http://www.scopus.com/inward/record.url?scp=85045657309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045657309&partnerID=8YFLogxK
U2 - 10.1117/12.2292125
DO - 10.1117/12.2292125
M3 - Conference contribution
VL - 10483
BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII
A2 - Tuchin, Valery V.
A2 - Tuchin, Valery V.
A2 - Fujimoto, James G.
A2 - Izatt, Joseph A.
PB - SPIE
ER -