Visibility enhancement of fluorescent objects hidden in animal tissues using spectral fluorescence difference method

W. B. Wang, S. G. Demos, J. Ali, G. Zhang, R. R. Alfano

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The difference between the fluorescence spectra emitted from Cardio Green dye and chicken breast tissue in the near-infrared (NIR) region was measured and used to select a pair of fingerprint wavelengths for imaging. Using spectral fluorescence difference imaging method, an 1-mm tissue object stained with Cardio Green dye located 3.1 cm underneath the surface of the host chicken tissue was clearly imaged and identified. The spatial resolution and the contrast of the fluorescence difference image were found to be significantly improved over the conventional single wavelength imaging approach.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalOptics Communications
Volume147
Issue number1-3
StatePublished - Feb 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Wang, W. B., Demos, S. G., Ali, J., Zhang, G., & Alfano, R. R. (1998). Visibility enhancement of fluorescent objects hidden in animal tissues using spectral fluorescence difference method. Optics Communications, 147(1-3), 11-15.