Viscoelastic eshelby analysis of cardiomyocyte contraction in the cell-in-gel system

Mohammad Kazemi-Lari, John Shaw, Alan Wineman, Rafael Shimkunas, Leighton T Izu, Ye Chen-Izu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a mathematical model to guide and interpret ongoing Cell-in-Gel experiments, where isolated cardiac myocytes are embedded in a constraining viscoelastic hydrogel, to study mechano-chemo-transduction mechanisms at the single cell level. A recently developed mathematical model, based on the elastic Eshelby inclusion problem, is here extended to account for viscoelasticity of the inclusion (cell) and the matrix (gel). This provides a tool to calculate time-dependent 3D stress and strain fields of a single myocyte contracting periodically inside a viscoelastic matrix, which is used to explore the sensitivity of the cell’s mechanical response to constitutive properties and geometry. A parametric study indicates that increased gel crosslink concentration significantly alters the strain and stress fields inside the cell and creates an increased time-lag in the mechanical response of the cell during contraction. It is also found that autoregulation at the cellular level in response to afterload, potentially in the form of increased cell stiffness, has a strong influence on cell contraction.

Original languageEnglish (US)
Title of host publicationMechanics and Behavior of Active Materials; Structural Health Monitoring; Bioinspired Smart Materials and Systems; Energy Harvesting; Emerging Technologies
PublisherAmerican Society of Mechanical Engineers (ASME)
Volume2
ISBN (Electronic)9780791851951
DOIs
StatePublished - Jan 1 2018
EventASME 2018 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS 2018 - San Antonio, United States
Duration: Sep 10 2018Sep 12 2018

Other

OtherASME 2018 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS 2018
CountryUnited States
CitySan Antonio
Period9/10/189/12/18

ASJC Scopus subject areas

  • Biomaterials
  • Civil and Structural Engineering

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    Kazemi-Lari, M., Shaw, J., Wineman, A., Shimkunas, R., Izu, L. T., & Chen-Izu, Y. (2018). Viscoelastic eshelby analysis of cardiomyocyte contraction in the cell-in-gel system. In Mechanics and Behavior of Active Materials; Structural Health Monitoring; Bioinspired Smart Materials and Systems; Energy Harvesting; Emerging Technologies (Vol. 2). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/SMASIS2018-8244