Visceral Smooth Muscle α-Actin Deficiency Associated With Chronic Intestinal Pseudo-obstruction in a Bengal Cat (Felis catus × Prionailurus bengalensis)

Denise Imai, J. L. Miller, Brian C Leonard, J. Bach, R. Drees, H. Steinberg, L. B.C. Teixeira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An adult Bengal cat (Felis catus × Prionailurus bengalensis) with a prolonged history of partial anorexia, regurgitation, and weight loss and a clinical, radiographic, and ultrasonographic diagnosis of persistent megaesophagus and gastrointestinal ileus was submitted for necropsy. The intestinal tract was diffusely distended by gas and fluid with appreciable loss of muscle tone and an absence of luminal obstruction, consistent with the clinical history of chronic intestinal pseudo-obstruction. Histologically, the autonomic nervous system was intact, but the smooth muscle within the gastrointestinal wall exhibited a marked basophilia that was most pronounced in the jejunum. Immunohistochemistry for neurofilament, synaptophysin, CD117, and desmin demonstrated that the number of myenteric ganglia, number of interstitial cells, and leiomyocyte desmin content were similar when compared with the unaffected age- and species-matched control. Immunohistochemistry for smooth muscle α-actin demonstrated a striking loss of immunoreactivity, predominantly in the circular layer of the jejunum, that corresponded with the tinctorial change in leiomyocytes. Transmission electron microscopy revealed loss of myofibrils, loss of organelle polarity, and significantly larger central mitochondria (megamitochondria) in affected leiomyocytes, as well as nonspecific degenerative changes. Although the presence of a primary leiomyopathy and a causal relationship could not be confirmed in this case, leiomyopathies are considered a cause of chronic intestinal pseudo-obstruction in human medicine, and loss of smooth muscle α-actin immunoreactivity is one recognized marker for intestinal dysmotility.

Original languageEnglish (US)
Pages (from-to)612-618
Number of pages7
JournalVeterinary Pathology
Volume51
Issue number3
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Prionailurus bengalensis
Intestinal Pseudo-Obstruction
intestinal obstruction
smooth muscle
actin
Smooth Muscle
Actins
Cats
Desmin
Jejunum
cats
desmin
jejunum
Immunohistochemistry
immunohistochemistry
Synaptophysin
Esophageal Achalasia
Intermediate Filaments
Ileus
Myofibrils

Keywords

  • chronic intestinal pseudo-obstruction
  • dysautonomia
  • feline
  • ileus
  • smooth muscle α-actin
  • visceral leiomyopathy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Visceral Smooth Muscle α-Actin Deficiency Associated With Chronic Intestinal Pseudo-obstruction in a Bengal Cat (Felis catus × Prionailurus bengalensis). / Imai, Denise; Miller, J. L.; Leonard, Brian C; Bach, J.; Drees, R.; Steinberg, H.; Teixeira, L. B.C.

In: Veterinary Pathology, Vol. 51, No. 3, 01.01.2014, p. 612-618.

Research output: Contribution to journalArticle

@article{d53628b947974b79b7b78f3ee37c9811,
title = "Visceral Smooth Muscle α-Actin Deficiency Associated With Chronic Intestinal Pseudo-obstruction in a Bengal Cat (Felis catus × Prionailurus bengalensis)",
abstract = "An adult Bengal cat (Felis catus × Prionailurus bengalensis) with a prolonged history of partial anorexia, regurgitation, and weight loss and a clinical, radiographic, and ultrasonographic diagnosis of persistent megaesophagus and gastrointestinal ileus was submitted for necropsy. The intestinal tract was diffusely distended by gas and fluid with appreciable loss of muscle tone and an absence of luminal obstruction, consistent with the clinical history of chronic intestinal pseudo-obstruction. Histologically, the autonomic nervous system was intact, but the smooth muscle within the gastrointestinal wall exhibited a marked basophilia that was most pronounced in the jejunum. Immunohistochemistry for neurofilament, synaptophysin, CD117, and desmin demonstrated that the number of myenteric ganglia, number of interstitial cells, and leiomyocyte desmin content were similar when compared with the unaffected age- and species-matched control. Immunohistochemistry for smooth muscle α-actin demonstrated a striking loss of immunoreactivity, predominantly in the circular layer of the jejunum, that corresponded with the tinctorial change in leiomyocytes. Transmission electron microscopy revealed loss of myofibrils, loss of organelle polarity, and significantly larger central mitochondria (megamitochondria) in affected leiomyocytes, as well as nonspecific degenerative changes. Although the presence of a primary leiomyopathy and a causal relationship could not be confirmed in this case, leiomyopathies are considered a cause of chronic intestinal pseudo-obstruction in human medicine, and loss of smooth muscle α-actin immunoreactivity is one recognized marker for intestinal dysmotility.",
keywords = "chronic intestinal pseudo-obstruction, dysautonomia, feline, ileus, smooth muscle α-actin, visceral leiomyopathy",
author = "Denise Imai and Miller, {J. L.} and Leonard, {Brian C} and J. Bach and R. Drees and H. Steinberg and Teixeira, {L. B.C.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1177/0300985813492802",
language = "English (US)",
volume = "51",
pages = "612--618",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Visceral Smooth Muscle α-Actin Deficiency Associated With Chronic Intestinal Pseudo-obstruction in a Bengal Cat (Felis catus × Prionailurus bengalensis)

AU - Imai, Denise

AU - Miller, J. L.

AU - Leonard, Brian C

AU - Bach, J.

AU - Drees, R.

AU - Steinberg, H.

AU - Teixeira, L. B.C.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - An adult Bengal cat (Felis catus × Prionailurus bengalensis) with a prolonged history of partial anorexia, regurgitation, and weight loss and a clinical, radiographic, and ultrasonographic diagnosis of persistent megaesophagus and gastrointestinal ileus was submitted for necropsy. The intestinal tract was diffusely distended by gas and fluid with appreciable loss of muscle tone and an absence of luminal obstruction, consistent with the clinical history of chronic intestinal pseudo-obstruction. Histologically, the autonomic nervous system was intact, but the smooth muscle within the gastrointestinal wall exhibited a marked basophilia that was most pronounced in the jejunum. Immunohistochemistry for neurofilament, synaptophysin, CD117, and desmin demonstrated that the number of myenteric ganglia, number of interstitial cells, and leiomyocyte desmin content were similar when compared with the unaffected age- and species-matched control. Immunohistochemistry for smooth muscle α-actin demonstrated a striking loss of immunoreactivity, predominantly in the circular layer of the jejunum, that corresponded with the tinctorial change in leiomyocytes. Transmission electron microscopy revealed loss of myofibrils, loss of organelle polarity, and significantly larger central mitochondria (megamitochondria) in affected leiomyocytes, as well as nonspecific degenerative changes. Although the presence of a primary leiomyopathy and a causal relationship could not be confirmed in this case, leiomyopathies are considered a cause of chronic intestinal pseudo-obstruction in human medicine, and loss of smooth muscle α-actin immunoreactivity is one recognized marker for intestinal dysmotility.

AB - An adult Bengal cat (Felis catus × Prionailurus bengalensis) with a prolonged history of partial anorexia, regurgitation, and weight loss and a clinical, radiographic, and ultrasonographic diagnosis of persistent megaesophagus and gastrointestinal ileus was submitted for necropsy. The intestinal tract was diffusely distended by gas and fluid with appreciable loss of muscle tone and an absence of luminal obstruction, consistent with the clinical history of chronic intestinal pseudo-obstruction. Histologically, the autonomic nervous system was intact, but the smooth muscle within the gastrointestinal wall exhibited a marked basophilia that was most pronounced in the jejunum. Immunohistochemistry for neurofilament, synaptophysin, CD117, and desmin demonstrated that the number of myenteric ganglia, number of interstitial cells, and leiomyocyte desmin content were similar when compared with the unaffected age- and species-matched control. Immunohistochemistry for smooth muscle α-actin demonstrated a striking loss of immunoreactivity, predominantly in the circular layer of the jejunum, that corresponded with the tinctorial change in leiomyocytes. Transmission electron microscopy revealed loss of myofibrils, loss of organelle polarity, and significantly larger central mitochondria (megamitochondria) in affected leiomyocytes, as well as nonspecific degenerative changes. Although the presence of a primary leiomyopathy and a causal relationship could not be confirmed in this case, leiomyopathies are considered a cause of chronic intestinal pseudo-obstruction in human medicine, and loss of smooth muscle α-actin immunoreactivity is one recognized marker for intestinal dysmotility.

KW - chronic intestinal pseudo-obstruction

KW - dysautonomia

KW - feline

KW - ileus

KW - smooth muscle α-actin

KW - visceral leiomyopathy

UR - http://www.scopus.com/inward/record.url?scp=84899458172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899458172&partnerID=8YFLogxK

U2 - 10.1177/0300985813492802

DO - 10.1177/0300985813492802

M3 - Article

VL - 51

SP - 612

EP - 618

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 3

ER -