Abstract
The close interactions of dogs with humans and surrounding wildlife provide frequent opportunities for cross-species virus transmissions. In order to initiate an unbiased characterization of the eukaryotic viruses in the gut of dogs, this study used deep sequencing of partially purified viral capsid-protected nucleic acids from the faeces of 18 diarrhoeic dogs. Known canine parvoviruses, coronaviruses and rotaviruses were identified, and the genomes of the first reported canine kobuvirus and sapovirus were characterized. Canine kobuvirus, the first sequenced canine picornavirus and the closest genetic relative of the diarrhoea-causing human Aichi virus, was detected at high frequency in the faeces of both healthy and diarrhoeic dogs. Canine sapovirus constituted a novel genogroup within the genus Sapovirus, a group of viruses also associated with human and animal diarrhoea. These results highlight the high frequency of new virus detection possible even in extensively studied animal species using metagenomics approaches, and provide viral genomes for further disease-association studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2534-2541 |
| Number of pages | 8 |
| Journal | Journal of General Virology |
| Volume | 92 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2011 |
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ASJC Scopus subject areas
- Virology
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Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses. / Li, Linlin; Pesavento, Patricia; Shan, Tongling; Leutenegger, Christian M.; Wang, Chunlin; Delwart, Eric.
In: Journal of General Virology, Vol. 92, No. 11, 11.2011, p. 2534-2541.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses
AU - Li, Linlin
AU - Pesavento, Patricia
AU - Shan, Tongling
AU - Leutenegger, Christian M.
AU - Wang, Chunlin
AU - Delwart, Eric
PY - 2011/11
Y1 - 2011/11
N2 - The close interactions of dogs with humans and surrounding wildlife provide frequent opportunities for cross-species virus transmissions. In order to initiate an unbiased characterization of the eukaryotic viruses in the gut of dogs, this study used deep sequencing of partially purified viral capsid-protected nucleic acids from the faeces of 18 diarrhoeic dogs. Known canine parvoviruses, coronaviruses and rotaviruses were identified, and the genomes of the first reported canine kobuvirus and sapovirus were characterized. Canine kobuvirus, the first sequenced canine picornavirus and the closest genetic relative of the diarrhoea-causing human Aichi virus, was detected at high frequency in the faeces of both healthy and diarrhoeic dogs. Canine sapovirus constituted a novel genogroup within the genus Sapovirus, a group of viruses also associated with human and animal diarrhoea. These results highlight the high frequency of new virus detection possible even in extensively studied animal species using metagenomics approaches, and provide viral genomes for further disease-association studies.
AB - The close interactions of dogs with humans and surrounding wildlife provide frequent opportunities for cross-species virus transmissions. In order to initiate an unbiased characterization of the eukaryotic viruses in the gut of dogs, this study used deep sequencing of partially purified viral capsid-protected nucleic acids from the faeces of 18 diarrhoeic dogs. Known canine parvoviruses, coronaviruses and rotaviruses were identified, and the genomes of the first reported canine kobuvirus and sapovirus were characterized. Canine kobuvirus, the first sequenced canine picornavirus and the closest genetic relative of the diarrhoea-causing human Aichi virus, was detected at high frequency in the faeces of both healthy and diarrhoeic dogs. Canine sapovirus constituted a novel genogroup within the genus Sapovirus, a group of viruses also associated with human and animal diarrhoea. These results highlight the high frequency of new virus detection possible even in extensively studied animal species using metagenomics approaches, and provide viral genomes for further disease-association studies.
UR - http://www.scopus.com/inward/record.url?scp=80053990304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053990304&partnerID=8YFLogxK
U2 - 10.1099/vir.0.034611-0
DO - 10.1099/vir.0.034611-0
M3 - Article
C2 - 21775584
AN - SCOPUS:80053990304
VL - 92
SP - 2534
EP - 2541
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 11
ER -