Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components

Nadejda Beliakova-Bethell, Carla Beckham, Thomas H. Giddings, Mark Winey, Roy Parker, Suzanne Sandmeyer

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and where genomic RNA are identified for retrovirus and retrotransposon assembly, and how translation and assembly processes are coordinated is poorly understood. To understand this process, the subcellular localization of Ty3 RNA and capsid proteins and virus-like particles was investigated. We demonstrate that mRNAs, proteins, and virus-like particles of the yeast Ty3 retrotransposon accumulate in association with cytoplasmic P-bodies, which are sites of mRNA translation repression, storage, and degradation. Deletions of genes encoding P-body proteins decreased Ty3 transposition and caused changes in the pattern of Ty3 foci, underscoring the biological significance of the association of Ty3 virus-like protein components and P-bodies. These results suggest the hypothesis that P-bodies may serve to segregate translation and assembly functions of the Ty3 genomic RNA to promote assembly of virus-like particles. Because Ty3 has features of a simple retrovirus and P-body functions are conserved between yeast and metazoan organisms, these findings may provide insights into host factors that facilitate retrovirus assembly. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)94-101
Number of pages8
JournalRNA
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Keywords

  • mRNA localization
  • P-body
  • Retrotransposon
  • Retrovirus
  • Ty3
  • Virus assembly

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components'. Together they form a unique fingerprint.

  • Cite this

    Beliakova-Bethell, N., Beckham, C., Giddings, T. H., Winey, M., Parker, R., & Sandmeyer, S. (2006). Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components. RNA, 12(1), 94-101. https://doi.org/10.1261/rna.2264806