Virus distribution and seasonal changes of grapevine leafroll-associated viruses

Fatima Osman, Deborah Golino, Emir Hodzic, Adib Rowhani

Research output: Contribution to journalArticle

Abstract

A time-course study was performed over two years to investigate seasonal changes in copy numbers of different Grapevine leafroll associated virus (GLRaV) in infected grapevine (Vitis vinifera) in North America. We examined GLRaV-1, -2, -3, -4, and GLRaV-2RG in 104 grapevines that previously tested positive for one or more of these GLRaVs. Vines were selected from the Davis grapevine virus collection and the USDA National Clonal Germplasm Repository in northern California. Samples were collected from the beginning of the growing season (late April) until February; from April to November, mature leaf petioles were collected, and from December to February, cambial scrapings were taken from dormant cuttings. All samples were tested by RT-PCR and RT-qPCR; RT-qPCR was found to be the more sensitive technique. GLRaVs were more reliably detected in samples collected between August and February. Grapevines infected with GLRaV-2 were evenly detected throughout the year, while infection with GLRaV-1, -3 -4, and -2RG was best observed from November to February for both experimental years. Virus titer was lowest early in the growing season (April/May), and viral load differed among the GLRaVs. The viral load of GLRaV-3 remained unchanged, regardless of the season tested. Viruses associated with leafroll disease in grapevines were not uniformly distributed in both leaf petioles and cambium of grapevines; GLRaV-2 was the most evenly distributed virus in the grapevine, followed by GLRaV-1, GLRaV-3, and finally by GLRaV-4, which had the most uneven distribution.

Original languageEnglish (US)
Pages (from-to)70-76
Number of pages7
JournalAmerican Journal of Enology and Viticulture
Volume69
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Grapevine leafroll-associated virus
Satellite Viruses
Grapevine leafroll-associated virus 1
Viruses
viruses
Grapevine leafroll-associated virus 2
viral load
Grapevine leafroll-associated virus 3
petioles
Viral Load
growing season
cambium
sampling
USDA
Vitis vinifera
Cambium
vines
leaves
germplasm
United States Department of Agriculture

Keywords

  • Grapevine leafroll viruses
  • Grapevine virus distribution
  • RT-qPCR
  • Sampling protocols
  • Seasonal changes
  • Time course

ASJC Scopus subject areas

  • Food Science
  • Horticulture

Cite this

Virus distribution and seasonal changes of grapevine leafroll-associated viruses. / Osman, Fatima; Golino, Deborah; Hodzic, Emir; Rowhani, Adib.

In: American Journal of Enology and Viticulture, Vol. 69, No. 1, 01.01.2018, p. 70-76.

Research output: Contribution to journalArticle

Osman, Fatima ; Golino, Deborah ; Hodzic, Emir ; Rowhani, Adib. / Virus distribution and seasonal changes of grapevine leafroll-associated viruses. In: American Journal of Enology and Viticulture. 2018 ; Vol. 69, No. 1. pp. 70-76.
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