Abstract
Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram- negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superoxide dismutase of Escherichia coli. All Salmonella serotypes appear to carry the sodCII locus, but the phage- associated sodCI gene is found only in certain strains belonging to the most highly pathogenic serotypes. Expression of either sodC locus appears to be enhanced during stationary phase, but only sodCII is regulated by the alternative sigma factor σ(s) (RpoS). Mutants lacking both sodC genes are less lethal for mice than mutants possessing either sodC locus alone, indicating that both Cu, Zn-SOD enzymes contribute to Salmonella pathogenicity. The evolutionary acquisition of an additional sodC gene has contributed to the enhanced virulence of selected Salmonella strains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7502-7507 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 96 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jun 22 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- General
Cite this
Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases. / Fang, Ferric C.; Degroote, Mary Ann; Foster, John W.; Baumler, Andreas J; Ochsner, Urs; Testerman, Traci; Bearson, Shawn; Giárd, Jean Christophe; Xu, Yisheng; Campbell, Gail; Laessig, Tracey.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 13, 22.06.1999, p. 7502-7507.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases
AU - Fang, Ferric C.
AU - Degroote, Mary Ann
AU - Foster, John W.
AU - Baumler, Andreas J
AU - Ochsner, Urs
AU - Testerman, Traci
AU - Bearson, Shawn
AU - Giárd, Jean Christophe
AU - Xu, Yisheng
AU - Campbell, Gail
AU - Laessig, Tracey
PY - 1999/6/22
Y1 - 1999/6/22
N2 - Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram- negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superoxide dismutase of Escherichia coli. All Salmonella serotypes appear to carry the sodCII locus, but the phage- associated sodCI gene is found only in certain strains belonging to the most highly pathogenic serotypes. Expression of either sodC locus appears to be enhanced during stationary phase, but only sodCII is regulated by the alternative sigma factor σ(s) (RpoS). Mutants lacking both sodC genes are less lethal for mice than mutants possessing either sodC locus alone, indicating that both Cu, Zn-SOD enzymes contribute to Salmonella pathogenicity. The evolutionary acquisition of an additional sodC gene has contributed to the enhanced virulence of selected Salmonella strains.
AB - Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram- negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superoxide dismutase of Escherichia coli. All Salmonella serotypes appear to carry the sodCII locus, but the phage- associated sodCI gene is found only in certain strains belonging to the most highly pathogenic serotypes. Expression of either sodC locus appears to be enhanced during stationary phase, but only sodCII is regulated by the alternative sigma factor σ(s) (RpoS). Mutants lacking both sodC genes are less lethal for mice than mutants possessing either sodC locus alone, indicating that both Cu, Zn-SOD enzymes contribute to Salmonella pathogenicity. The evolutionary acquisition of an additional sodC gene has contributed to the enhanced virulence of selected Salmonella strains.
UR - http://www.scopus.com/inward/record.url?scp=13044300853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13044300853&partnerID=8YFLogxK
U2 - 10.1073/pnas.96.13.7502
DO - 10.1073/pnas.96.13.7502
M3 - Article
C2 - 10377444
AN - SCOPUS:13044300853
VL - 96
SP - 7502
EP - 7507
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 13
ER -