Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases

Ferric C. Fang, Mary Ann Degroote, John W. Foster, Andreas J Baumler, Urs Ochsner, Traci Testerman, Shawn Bearson, Jean Christophe Giárd, Yisheng Xu, Gail Campbell, Tracey Laessig

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram- negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superoxide dismutase of Escherichia coli. All Salmonella serotypes appear to carry the sodCII locus, but the phage- associated sodCI gene is found only in certain strains belonging to the most highly pathogenic serotypes. Expression of either sodC locus appears to be enhanced during stationary phase, but only sodCII is regulated by the alternative sigma factor σ(s) (RpoS). Mutants lacking both sodC genes are less lethal for mice than mutants possessing either sodC locus alone, indicating that both Cu, Zn-SOD enzymes contribute to Salmonella pathogenicity. The evolutionary acquisition of an additional sodC gene has contributed to the enhanced virulence of selected Salmonella strains.

Original languageEnglish (US)
Pages (from-to)7502-7507
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number13
DOIs
StatePublished - Jun 22 1999
Externally publishedYes

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Salmonella typhimurium
Salmonella
Bacteriophages
Virulence
Lethal Genes
Sigma Factor
Enzymes
Gram-Negative Bacteria
Genes
Escherichia coli
Amino Acids
Superoxide Dismutase-1
Serogroup

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases. / Fang, Ferric C.; Degroote, Mary Ann; Foster, John W.; Baumler, Andreas J; Ochsner, Urs; Testerman, Traci; Bearson, Shawn; Giárd, Jean Christophe; Xu, Yisheng; Campbell, Gail; Laessig, Tracey.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 13, 22.06.1999, p. 7502-7507.

Research output: Contribution to journalArticle

Fang, FC, Degroote, MA, Foster, JW, Baumler, AJ, Ochsner, U, Testerman, T, Bearson, S, Giárd, JC, Xu, Y, Campbell, G & Laessig, T 1999, 'Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases', Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 13, pp. 7502-7507. https://doi.org/10.1073/pnas.96.13.7502
Fang, Ferric C. ; Degroote, Mary Ann ; Foster, John W. ; Baumler, Andreas J ; Ochsner, Urs ; Testerman, Traci ; Bearson, Shawn ; Giárd, Jean Christophe ; Xu, Yisheng ; Campbell, Gail ; Laessig, Tracey. / Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 13. pp. 7502-7507.
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