Virulence of Serovar C-1 Strains of Avibacterium paragallinarum

H. H. Trujillo-Ruíz, H L Shivaprasad, V. Morales-Erasto, M. Talavera-Rojas, C. Salgado-Miranda, F. Salazar-García, P. J. Blackall, E. Soriano-Vargas

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

SUMMARY The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. There are nine serovars of A. paragallinarum, and serovar C-1 has emerged in outbreaks of infectious coryza in layer hens in the Americas, with all isolates having been obtained from infectious coryza-vaccinated chickens. In the current study, the clinical and histopathologic outcomes of experimental infections in chickens with A. paragallinarum of serovar C-1 were investigated. The Japanese serovar reference strain, H-18, and a Mexican isolate, ESV-135, were included in the study. No differences in clinical sign scores or morbidity were observed between the two strains. The two bacterial strains caused microscopic lesions of lymphoplasmacytic inflammation in the mucosa of the nasal cavity, infraorbital sinus, and trachea. Similar severe lesions were observed in birds inoculated with both H-18 and ESV-135 strains. The lesions were present 48 hr after inoculation and persisted until day 10 after inoculation. Slight to severe, extensive hemorrhages were observed in the lumen, mucous membranes, and lamina propria of the nasal cavity and infraorbital sinus in most of the chickens inoculated with either the reference strain H-18 or the ESV-135 isolate. Hemorrhages in the upper respiratory tract of chickens experimentally infected with A. paragallinarum are reported here for the first time. The results have confirmed the high virulence of the reference strain H-18 as previously reported and have shown that the Mexican isolate was as virulent as the reference strain. The virulence of A. paragallinarum isolates may play a role in explaining why severe infectious coryza outbreaks are being seen in both vaccinated and nonvaccinated chicken flocks.

Original languageEnglish (US)
Pages (from-to)837-840
Number of pages4
JournalAvian Diseases
Volume60
Issue number4
DOIs
StatePublished - Dec 1 2016

Fingerprint

Avibacterium paragallinarum
Virulence
Chickens
serotypes
virulence
chickens
lesions (animal)
Mucous Membrane
Nasal Cavity
nasal cavity
Disease Outbreaks
sinuses
mucosa
hemorrhage
Hemorrhage
trachea (vertebrates)
Trachea
laminae (animals)
Respiratory System
Birds

Keywords

  • Avibacterium paragallinarum
  • infectious coryza
  • poultry
  • serovar C-1
  • virulence

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Trujillo-Ruíz, H. H., Shivaprasad, H. L., Morales-Erasto, V., Talavera-Rojas, M., Salgado-Miranda, C., Salazar-García, F., ... Soriano-Vargas, E. (2016). Virulence of Serovar C-1 Strains of Avibacterium paragallinarum. Avian Diseases, 60(4), 837-840. https://doi.org/10.1637/11421-040716-ResNote

Virulence of Serovar C-1 Strains of Avibacterium paragallinarum. / Trujillo-Ruíz, H. H.; Shivaprasad, H L; Morales-Erasto, V.; Talavera-Rojas, M.; Salgado-Miranda, C.; Salazar-García, F.; Blackall, P. J.; Soriano-Vargas, E.

In: Avian Diseases, Vol. 60, No. 4, 01.12.2016, p. 837-840.

Research output: Contribution to journalArticle

Trujillo-Ruíz, HH, Shivaprasad, HL, Morales-Erasto, V, Talavera-Rojas, M, Salgado-Miranda, C, Salazar-García, F, Blackall, PJ & Soriano-Vargas, E 2016, 'Virulence of Serovar C-1 Strains of Avibacterium paragallinarum', Avian Diseases, vol. 60, no. 4, pp. 837-840. https://doi.org/10.1637/11421-040716-ResNote
Trujillo-Ruíz HH, Shivaprasad HL, Morales-Erasto V, Talavera-Rojas M, Salgado-Miranda C, Salazar-García F et al. Virulence of Serovar C-1 Strains of Avibacterium paragallinarum. Avian Diseases. 2016 Dec 1;60(4):837-840. https://doi.org/10.1637/11421-040716-ResNote
Trujillo-Ruíz, H. H. ; Shivaprasad, H L ; Morales-Erasto, V. ; Talavera-Rojas, M. ; Salgado-Miranda, C. ; Salazar-García, F. ; Blackall, P. J. ; Soriano-Vargas, E. / Virulence of Serovar C-1 Strains of Avibacterium paragallinarum. In: Avian Diseases. 2016 ; Vol. 60, No. 4. pp. 837-840.
@article{365bdf493b8c413cbe081228f9005d94,
title = "Virulence of Serovar C-1 Strains of Avibacterium paragallinarum",
abstract = "SUMMARY The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. There are nine serovars of A. paragallinarum, and serovar C-1 has emerged in outbreaks of infectious coryza in layer hens in the Americas, with all isolates having been obtained from infectious coryza-vaccinated chickens. In the current study, the clinical and histopathologic outcomes of experimental infections in chickens with A. paragallinarum of serovar C-1 were investigated. The Japanese serovar reference strain, H-18, and a Mexican isolate, ESV-135, were included in the study. No differences in clinical sign scores or morbidity were observed between the two strains. The two bacterial strains caused microscopic lesions of lymphoplasmacytic inflammation in the mucosa of the nasal cavity, infraorbital sinus, and trachea. Similar severe lesions were observed in birds inoculated with both H-18 and ESV-135 strains. The lesions were present 48 hr after inoculation and persisted until day 10 after inoculation. Slight to severe, extensive hemorrhages were observed in the lumen, mucous membranes, and lamina propria of the nasal cavity and infraorbital sinus in most of the chickens inoculated with either the reference strain H-18 or the ESV-135 isolate. Hemorrhages in the upper respiratory tract of chickens experimentally infected with A. paragallinarum are reported here for the first time. The results have confirmed the high virulence of the reference strain H-18 as previously reported and have shown that the Mexican isolate was as virulent as the reference strain. The virulence of A. paragallinarum isolates may play a role in explaining why severe infectious coryza outbreaks are being seen in both vaccinated and nonvaccinated chicken flocks.",
keywords = "Avibacterium paragallinarum, infectious coryza, poultry, serovar C-1, virulence",
author = "Trujillo-Ru{\'i}z, {H. H.} and Shivaprasad, {H L} and V. Morales-Erasto and M. Talavera-Rojas and C. Salgado-Miranda and F. Salazar-Garc{\'i}a and Blackall, {P. J.} and E. Soriano-Vargas",
year = "2016",
month = "12",
day = "1",
doi = "10.1637/11421-040716-ResNote",
language = "English (US)",
volume = "60",
pages = "837--840",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "4",

}

TY - JOUR

T1 - Virulence of Serovar C-1 Strains of Avibacterium paragallinarum

AU - Trujillo-Ruíz, H. H.

AU - Shivaprasad, H L

AU - Morales-Erasto, V.

AU - Talavera-Rojas, M.

AU - Salgado-Miranda, C.

AU - Salazar-García, F.

AU - Blackall, P. J.

AU - Soriano-Vargas, E.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - SUMMARY The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. There are nine serovars of A. paragallinarum, and serovar C-1 has emerged in outbreaks of infectious coryza in layer hens in the Americas, with all isolates having been obtained from infectious coryza-vaccinated chickens. In the current study, the clinical and histopathologic outcomes of experimental infections in chickens with A. paragallinarum of serovar C-1 were investigated. The Japanese serovar reference strain, H-18, and a Mexican isolate, ESV-135, were included in the study. No differences in clinical sign scores or morbidity were observed between the two strains. The two bacterial strains caused microscopic lesions of lymphoplasmacytic inflammation in the mucosa of the nasal cavity, infraorbital sinus, and trachea. Similar severe lesions were observed in birds inoculated with both H-18 and ESV-135 strains. The lesions were present 48 hr after inoculation and persisted until day 10 after inoculation. Slight to severe, extensive hemorrhages were observed in the lumen, mucous membranes, and lamina propria of the nasal cavity and infraorbital sinus in most of the chickens inoculated with either the reference strain H-18 or the ESV-135 isolate. Hemorrhages in the upper respiratory tract of chickens experimentally infected with A. paragallinarum are reported here for the first time. The results have confirmed the high virulence of the reference strain H-18 as previously reported and have shown that the Mexican isolate was as virulent as the reference strain. The virulence of A. paragallinarum isolates may play a role in explaining why severe infectious coryza outbreaks are being seen in both vaccinated and nonvaccinated chicken flocks.

AB - SUMMARY The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. There are nine serovars of A. paragallinarum, and serovar C-1 has emerged in outbreaks of infectious coryza in layer hens in the Americas, with all isolates having been obtained from infectious coryza-vaccinated chickens. In the current study, the clinical and histopathologic outcomes of experimental infections in chickens with A. paragallinarum of serovar C-1 were investigated. The Japanese serovar reference strain, H-18, and a Mexican isolate, ESV-135, were included in the study. No differences in clinical sign scores or morbidity were observed between the two strains. The two bacterial strains caused microscopic lesions of lymphoplasmacytic inflammation in the mucosa of the nasal cavity, infraorbital sinus, and trachea. Similar severe lesions were observed in birds inoculated with both H-18 and ESV-135 strains. The lesions were present 48 hr after inoculation and persisted until day 10 after inoculation. Slight to severe, extensive hemorrhages were observed in the lumen, mucous membranes, and lamina propria of the nasal cavity and infraorbital sinus in most of the chickens inoculated with either the reference strain H-18 or the ESV-135 isolate. Hemorrhages in the upper respiratory tract of chickens experimentally infected with A. paragallinarum are reported here for the first time. The results have confirmed the high virulence of the reference strain H-18 as previously reported and have shown that the Mexican isolate was as virulent as the reference strain. The virulence of A. paragallinarum isolates may play a role in explaining why severe infectious coryza outbreaks are being seen in both vaccinated and nonvaccinated chicken flocks.

KW - Avibacterium paragallinarum

KW - infectious coryza

KW - poultry

KW - serovar C-1

KW - virulence

UR - http://www.scopus.com/inward/record.url?scp=85002369819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85002369819&partnerID=8YFLogxK

U2 - 10.1637/11421-040716-ResNote

DO - 10.1637/11421-040716-ResNote

M3 - Article

VL - 60

SP - 837

EP - 840

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 4

ER -