Virome of > 12 thousand Culex mosquitoes from throughout California

Mohammadreza Sadeghi, Eda Altan, Xutao Deng, Chris Barker, Ying Fang, Lark L Schneider, Eric Delwart

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metagenomic analysis of whole mosquitoes allows the genetic characterization of all associated viruses, including arboviruses and insect-specific viruses, plus those in their diet or infecting their parasites. We describe here the virome in mosquitoes, primarily Culex pipiens complex, Cx. tarsalis and Cx. erythrothorax, collected in 2016 from 31 counties in California, USA. The nearly complete genomes of 56 viruses, including 32 novel genomes, some from potentially novel RNA and DNA viral families or genera, were assembled and phylogenetically analyzed, significantly expanding the known Culex-associated virome. The majority of detected viral sequences originated from single-stranded RNA viral families with members known to infect insects, plants, or from unknown hosts. These reference viral genomes will facilitate the identification of related viruses in other insect species and to monitor changes in the virome of Culex mosquito populations to define factors influencing their transmission and possible impact on their insect hosts.

Original languageEnglish (US)
Pages (from-to)74-88
Number of pages15
JournalVirology
Volume523
DOIs
StatePublished - Oct 1 2018

Fingerprint

Culex
Culicidae
Insects
Viral RNA
Insect Viruses
Genome
Viruses
Satellite Viruses
Arboviruses
Metagenomics
Viral Genome
Viral DNA
Parasites
Diet
Population

Keywords

  • Bunyaviridae, Rhabdoviridae
  • Culex
  • Dicistroviridae
  • Flaviviridae
  • Iflaviviridae
  • Metagenomics
  • Mosquito
  • NGS
  • Virome

ASJC Scopus subject areas

  • Virology

Cite this

Virome of > 12 thousand Culex mosquitoes from throughout California. / Sadeghi, Mohammadreza; Altan, Eda; Deng, Xutao; Barker, Chris; Fang, Ying; Schneider, Lark L; Delwart, Eric.

In: Virology, Vol. 523, 01.10.2018, p. 74-88.

Research output: Contribution to journalArticle

Sadeghi, Mohammadreza ; Altan, Eda ; Deng, Xutao ; Barker, Chris ; Fang, Ying ; Schneider, Lark L ; Delwart, Eric. / Virome of > 12 thousand Culex mosquitoes from throughout California. In: Virology. 2018 ; Vol. 523. pp. 74-88.
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