Viremia and serological responses in adult chickens infected with western equine encephalomyelitis and St. Louis encephalitis viruses.

W. K. Reisen, S. B. Presser, J. Lin, B. Enge, J. L. Hardy, R. W. Emmons

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Adult hens, similar to those used for arbovirus surveillance, were experimentally infected with western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses to describe the viremia response, to compare serological testing methods, and to evaluate a new method of collecting whole blood onto filter paper strips from lancet pricks of the chicken comb. Young (19 weeks), but not old (38 weeks), hens developed a low-titer, transient viremia for a 1-day period. Immunoglobulin G (IgG) was detected by days 10 and 14 after infection with WEE and SLE viruses, respectively, by indirect fluorescent antibody tests, hemagglutination inhibition tests, and plaque reduction neutralization tests on sera and in direct enzyme immunoassays (EIA) on both sera and eluates from filter paper samples. Immunoglobulin M (IgM) was first detected in sera 2 and 3 days before IgG, respectively, but IgM could not be detected reliably in eluates from dried blood. Sera and dried blood samples collected from naturally infected sentinel chickens gave comparable results when tested by an EIA for IgG.

Original languageEnglish (US)
Pages (from-to)549-555
Number of pages7
JournalJournal of the American Mosquito Control Association
Volume10
Issue number4
StatePublished - Dec 1 1994

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Public Health, Environmental and Occupational Health
  • Insect Science

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