Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells

Branko Vukosavljevic, Marius Hittinger, Henning Hachmeister, Christian Pilger, Xabier Murgia, Michael M. Gepp, Luca Gentile, Hanno Huwer, Nicole Schneider-Daum, Thomas R Huser, Claus Michael Lehr, Maike Windbergs

Research output: Contribution to journalEditorial

1 Scopus citations

Abstract

Alveolar type II (ATII) cells in the peripheral human lung spontaneously differentiate toward ATI cells, thus enabling air-blood barrier formation. Here, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy are applied to study cell differentiation of freshly isolated ATII cells. The Raman spectra can successfully be correlated with gradual morphological and molecular changes during cell differentiation. Alveolar surfactant rich vesicles in ATII cells are identified based on phospholipid vibrations, while ATI-like cells are characterized by the absence of vesicular structures. Complementary, CARS microscopy allows for three-dimensional visualization of lipid vesicles within ATII cells and their secretion, while hyperspectral CARS enables the distinction between cellular proteins and lipids according to their vibrational signatures. This study paves the path for further label-free investigations of lung cells and the role of the pulmonary surfactant, thus also providing a basis for rational development of future lung therapeutics.

Original languageEnglish (US)
Article numbere201800052
JournalJournal of Biophotonics
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

Keywords

  • CARS microcopy
  • cell imaging
  • confocal laser scanning microscopy
  • confocal Raman microscopy
  • pneumocyte type II differentiation

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells'. Together they form a unique fingerprint.

  • Cite this

    Vukosavljevic, B., Hittinger, M., Hachmeister, H., Pilger, C., Murgia, X., Gepp, M. M., Gentile, L., Huwer, H., Schneider-Daum, N., Huser, T. R., Lehr, C. M., & Windbergs, M. (2019). Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells. Journal of Biophotonics, 12(6), [e201800052]. https://doi.org/10.1002/jbio.201800052