Viability and functionality of cells delivered from peptide conjugated scaffolds

Voranaddha Vacharathit, Eduardo Silva, David J. Mooney

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Many cell-based therapies aim to transplant functional cells to revascularize damaged tissues and ischemic areas. However, conventional cell therapy is not optimally efficient: massive cell death, damage, and non-localization of cells both spatially and temporally all likely contribute to poor tissue functionality. An alginate cell depot system has been proposed as an alternative means to deliver outgrowth endothelial cells (OECs) in a spatiotemporally controllable manner while protecting them in the early stages of tissue re-integration. Here OECs exiting the alginate scaffold were measured for viability, functionality, and migration speed and characterized for cytokine and surface marker profiles. OECs were highly viable in the alginate and were depleted from the scaffold via migration at a speed of 21 ± 6 μm/h following release. Prolonged interaction with the alginate scaffold microenvironment did not detrimentally change OECs; they retained high functionality, displayed a similar angiogenic cytokine profile as control OECs, and did not have significantly altered surface markers. These results suggest that alginate-OEC interactions do not adversely affect these cells, validating control of cellular migration as a means to control the cell delivery profile from the material system, and supporting usage of the alginate scaffold as an efficient cell delivery vehicle.

Original languageEnglish (US)
Pages (from-to)3721-3728
Number of pages8
JournalBiomaterials
Volume32
Issue number15
DOIs
StatePublished - May 1 2011
Externally publishedYes

Fingerprint

Alginate
Endothelial cells
Scaffolds (biology)
Scaffolds
Peptides
Cell Survival
Endothelial Cells
Tissue
Cell- and Tissue-Based Therapy
Cytokines
Transplants
Cell death
Cell Communication
alginic acid
Cell Death

Keywords

  • Alginate
  • Angiogenesis
  • Biomimetic material
  • Cell activation
  • Cell adhesion
  • RGD peptide

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Viability and functionality of cells delivered from peptide conjugated scaffolds. / Vacharathit, Voranaddha; Silva, Eduardo; Mooney, David J.

In: Biomaterials, Vol. 32, No. 15, 01.05.2011, p. 3721-3728.

Research output: Contribution to journalArticle

Vacharathit, Voranaddha ; Silva, Eduardo ; Mooney, David J. / Viability and functionality of cells delivered from peptide conjugated scaffolds. In: Biomaterials. 2011 ; Vol. 32, No. 15. pp. 3721-3728.
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