Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane

Jun Lin Liao, Jia Chen, Bin He, Yong Chen, Jia Qun Xu, Hong Ju Xie, Feng Hu, Aijun Wang, Chengqun Luo, Qing Feng Li, Jian Da Zhou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

s: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. Methods: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane. Platelet-rich plasma was prepared using 8 mL of auricular blood. Three subcutaneous pockets were made in the backs of the rabbits, and the grafts were placed in these pockets. The subcutaneous implant tests were conducted for safety assessment of the PLGA membrane in vivo. All of the rabbits were sacrificed at the end of 3 months, and the specimens were collected. The sections were stained with hematoxylin and eosin, toluidin blue, and collagen II immunohistochemical. Simultaneously, biomechanical properties of grafts were assessed. Results: This sample of PLGA membrane was conformed to the current standard of biological evaluation of medical devices. Moderate resorption was seen at the end of 3 months in the gross assessment in diced cartilage wrapped with PLGA membrane, while diced cartilage blended with PRP had no apparent resorption macroscopically and favorable viability in vivo after 3 months, and the histological parameters supported this. Stress-strain curves for the compression test indicated that the modulus of elasticity of bare diced cartilage was 7.65 ± 0.59 MPa; diced cartilage wrapped with PLGA membrane was 5.98 ± 0.45 MPa; and diced cartilage blended with PRP and wrapped with PLGA membrane was 7.48 ± 0.55 MPa, respectively. Conclusions: Diced cartilage wrapped with PLGA membrane had moderate resorption macroscopically after 3 months. However, blending with PRP has beneficial effects in improving the viability of diced cartilages. Additionally, the compression modulus of diced cartilage blended with PRP and wrapped with PLGA membrane was similar to bare diced cartilage.

Original languageEnglish (US)
Pages (from-to)1418-1424
Number of pages7
JournalJournal of Craniofacial Surgery
Volume28
Issue number6
DOIs
StatePublished - Sep 1 2017

Fingerprint

Platelet-Rich Plasma
Biomechanical Phenomena
Cartilage
Membranes
Rabbits
Transplants
polylactic acid-polyglycolic acid copolymer
Elastic Modulus
Hematoxylin
Eosine Yellowish-(YS)
Ear

Keywords

  • Biomechanics
  • diced cartilage
  • platelet-rich plasma
  • PLGA
  • viability

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane. / Liao, Jun Lin; Chen, Jia; He, Bin; Chen, Yong; Xu, Jia Qun; Xie, Hong Ju; Hu, Feng; Wang, Aijun; Luo, Chengqun; Li, Qing Feng; Zhou, Jian Da.

In: Journal of Craniofacial Surgery, Vol. 28, No. 6, 01.09.2017, p. 1418-1424.

Research output: Contribution to journalArticle

Liao, Jun Lin ; Chen, Jia ; He, Bin ; Chen, Yong ; Xu, Jia Qun ; Xie, Hong Ju ; Hu, Feng ; Wang, Aijun ; Luo, Chengqun ; Li, Qing Feng ; Zhou, Jian Da. / Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane. In: Journal of Craniofacial Surgery. 2017 ; Vol. 28, No. 6. pp. 1418-1424.
@article{12bdc55f8f8f40ed8dc45bbe4460701d,
title = "Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane",
abstract = "s: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. Methods: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane. Platelet-rich plasma was prepared using 8 mL of auricular blood. Three subcutaneous pockets were made in the backs of the rabbits, and the grafts were placed in these pockets. The subcutaneous implant tests were conducted for safety assessment of the PLGA membrane in vivo. All of the rabbits were sacrificed at the end of 3 months, and the specimens were collected. The sections were stained with hematoxylin and eosin, toluidin blue, and collagen II immunohistochemical. Simultaneously, biomechanical properties of grafts were assessed. Results: This sample of PLGA membrane was conformed to the current standard of biological evaluation of medical devices. Moderate resorption was seen at the end of 3 months in the gross assessment in diced cartilage wrapped with PLGA membrane, while diced cartilage blended with PRP had no apparent resorption macroscopically and favorable viability in vivo after 3 months, and the histological parameters supported this. Stress-strain curves for the compression test indicated that the modulus of elasticity of bare diced cartilage was 7.65 ± 0.59 MPa; diced cartilage wrapped with PLGA membrane was 5.98 ± 0.45 MPa; and diced cartilage blended with PRP and wrapped with PLGA membrane was 7.48 ± 0.55 MPa, respectively. Conclusions: Diced cartilage wrapped with PLGA membrane had moderate resorption macroscopically after 3 months. However, blending with PRP has beneficial effects in improving the viability of diced cartilages. Additionally, the compression modulus of diced cartilage blended with PRP and wrapped with PLGA membrane was similar to bare diced cartilage.",
keywords = "Biomechanics, diced cartilage, platelet-rich plasma, PLGA, viability",
author = "Liao, {Jun Lin} and Jia Chen and Bin He and Yong Chen and Xu, {Jia Qun} and Xie, {Hong Ju} and Feng Hu and Aijun Wang and Chengqun Luo and Li, {Qing Feng} and Zhou, {Jian Da}",
year = "2017",
month = "9",
day = "1",
doi = "10.1097/SCS.0000000000003739",
language = "English (US)",
volume = "28",
pages = "1418--1424",
journal = "Journal of Craniofacial Surgery",
issn = "1049-2275",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Viability and Biomechanics of Diced Cartilage Blended with Platelet-Rich Plasma and Wrapped with Poly (Lactic-Co-Glycolic) Acid Membrane

AU - Liao, Jun Lin

AU - Chen, Jia

AU - He, Bin

AU - Chen, Yong

AU - Xu, Jia Qun

AU - Xie, Hong Ju

AU - Hu, Feng

AU - Wang, Aijun

AU - Luo, Chengqun

AU - Li, Qing Feng

AU - Zhou, Jian Da

PY - 2017/9/1

Y1 - 2017/9/1

N2 - s: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. Methods: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane. Platelet-rich plasma was prepared using 8 mL of auricular blood. Three subcutaneous pockets were made in the backs of the rabbits, and the grafts were placed in these pockets. The subcutaneous implant tests were conducted for safety assessment of the PLGA membrane in vivo. All of the rabbits were sacrificed at the end of 3 months, and the specimens were collected. The sections were stained with hematoxylin and eosin, toluidin blue, and collagen II immunohistochemical. Simultaneously, biomechanical properties of grafts were assessed. Results: This sample of PLGA membrane was conformed to the current standard of biological evaluation of medical devices. Moderate resorption was seen at the end of 3 months in the gross assessment in diced cartilage wrapped with PLGA membrane, while diced cartilage blended with PRP had no apparent resorption macroscopically and favorable viability in vivo after 3 months, and the histological parameters supported this. Stress-strain curves for the compression test indicated that the modulus of elasticity of bare diced cartilage was 7.65 ± 0.59 MPa; diced cartilage wrapped with PLGA membrane was 5.98 ± 0.45 MPa; and diced cartilage blended with PRP and wrapped with PLGA membrane was 7.48 ± 0.55 MPa, respectively. Conclusions: Diced cartilage wrapped with PLGA membrane had moderate resorption macroscopically after 3 months. However, blending with PRP has beneficial effects in improving the viability of diced cartilages. Additionally, the compression modulus of diced cartilage blended with PRP and wrapped with PLGA membrane was similar to bare diced cartilage.

AB - s: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. Methods: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane. Platelet-rich plasma was prepared using 8 mL of auricular blood. Three subcutaneous pockets were made in the backs of the rabbits, and the grafts were placed in these pockets. The subcutaneous implant tests were conducted for safety assessment of the PLGA membrane in vivo. All of the rabbits were sacrificed at the end of 3 months, and the specimens were collected. The sections were stained with hematoxylin and eosin, toluidin blue, and collagen II immunohistochemical. Simultaneously, biomechanical properties of grafts were assessed. Results: This sample of PLGA membrane was conformed to the current standard of biological evaluation of medical devices. Moderate resorption was seen at the end of 3 months in the gross assessment in diced cartilage wrapped with PLGA membrane, while diced cartilage blended with PRP had no apparent resorption macroscopically and favorable viability in vivo after 3 months, and the histological parameters supported this. Stress-strain curves for the compression test indicated that the modulus of elasticity of bare diced cartilage was 7.65 ± 0.59 MPa; diced cartilage wrapped with PLGA membrane was 5.98 ± 0.45 MPa; and diced cartilage blended with PRP and wrapped with PLGA membrane was 7.48 ± 0.55 MPa, respectively. Conclusions: Diced cartilage wrapped with PLGA membrane had moderate resorption macroscopically after 3 months. However, blending with PRP has beneficial effects in improving the viability of diced cartilages. Additionally, the compression modulus of diced cartilage blended with PRP and wrapped with PLGA membrane was similar to bare diced cartilage.

KW - Biomechanics

KW - diced cartilage

KW - platelet-rich plasma

KW - PLGA

KW - viability

UR - http://www.scopus.com/inward/record.url?scp=85022227722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85022227722&partnerID=8YFLogxK

U2 - 10.1097/SCS.0000000000003739

DO - 10.1097/SCS.0000000000003739

M3 - Article

C2 - 28692513

AN - SCOPUS:85022227722

VL - 28

SP - 1418

EP - 1424

JO - Journal of Craniofacial Surgery

JF - Journal of Craniofacial Surgery

SN - 1049-2275

IS - 6

ER -