Vesicular glutamate transporters define two sets of glutamatergic afferents to the somatosensory thalamus and two thalamocortical projections in the mouse

Alessandro Graziano, Xiao Bo Liu, Karl D Murray, Edward G. Jones

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The ventral posterior nucleus of the thalamus (VP) receives two major sets of excitatory inputs, one from the ascending somatosensory pathways originating in the dorsal horn, dorsal column nuclei, and trigeminal nuclei, and the other originating from the cerebral cortex. Both systems use glutamate as neurotransmitter, as do the thalamocortical axons relaying somatosensory information from the VP to the primary somatosensory cortex (SI). The synapses formed by these projection systems differ anatomically, physiologically, and in their capacity for short-term synaptic plasticity. Glutamate uptake into synaptic vesicles and its release at central synapses depend on two isoforms of vesicular glutamate transporters, VGluT1 and VGluT2. Despite ample evidence of their complementary distribution, some instances exist of co-localization in the same brain areas or at the same synapses. In the thalamus, the two transcripts coexist in cells of the VP and other nuclei but not in the posterior or intralaminar nuclei. We show that the two isoforms are completely segregated at VP synapses, despite their widespread expression throughout the dorsal and ventral thalamus. We present immunocytochemical, ultrastructural, gene expression, and connectional evidence that VGluT1 in the VP is only found at corticothalamic synapses, whereas VGluT2 is only found at terminals made by axons originating in the spinal cord and brainstem. By contrast, the two VGluT isoforms are co-localized in thalamocortical axon terminals targeting layer IV, but not in those targeting layer I, suggesting the presence of two distinct projection systems related to the core/matrix pattern of organization of thalamocortical connectivity described in other mammals.

Original languageEnglish (US)
Pages (from-to)1258-1276
Number of pages19
JournalJournal of Comparative Neurology
Volume507
Issue number2
DOIs
StatePublished - Mar 10 2008

Fingerprint

Vesicular Glutamate Transport Proteins
Thalamus
Synapses
Ventral Thalamic Nuclei
Protein Isoforms
Presynaptic Terminals
Glutamic Acid
Posterior Thalamic Nuclei
Trigeminal Nuclei
Neuronal Plasticity
Somatosensory Cortex
Synaptic Vesicles
Cerebral Cortex
Brain Stem
Neurotransmitter Agents
Axons
Mammals
Spinal Cord
Gene Expression
Brain

Keywords

  • BNPI
  • Core-matrix
  • Corticothalamic
  • DNPI
  • Medial lemniscus
  • Spinothalamic
  • Synapses

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Vesicular glutamate transporters define two sets of glutamatergic afferents to the somatosensory thalamus and two thalamocortical projections in the mouse. / Graziano, Alessandro; Liu, Xiao Bo; Murray, Karl D; Jones, Edward G.

In: Journal of Comparative Neurology, Vol. 507, No. 2, 10.03.2008, p. 1258-1276.

Research output: Contribution to journalArticle

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