Abstract
This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-197 |
| Number of pages | 15 |
| Journal | Brain Research |
| Volume | 1234 |
| DOIs | |
| State | Published - Oct 9 2008 |
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Keywords
- Brain
- Heat shock protein
- Infarction
- Ischemia
- Microglia
- Transient ischemic attacks (TIAs)
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein. / Zhan, Xinhua; Kim, Charles; Sharp, Frank R.
In: Brain Research, Vol. 1234, 09.10.2008, p. 183-197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein
AU - Zhan, Xinhua
AU - Kim, Charles
AU - Sharp, Frank R
PY - 2008/10/9
Y1 - 2008/10/9
N2 - This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.
AB - This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.
KW - Brain
KW - Heat shock protein
KW - Infarction
KW - Ischemia
KW - Microglia
KW - Transient ischemic attacks (TIAs)
UR - http://www.scopus.com/inward/record.url?scp=52049112072&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52049112072&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2008.07.094
DO - 10.1016/j.brainres.2008.07.094
M3 - Article
C2 - 18708034
AN - SCOPUS:52049112072
VL - 1234
SP - 183
EP - 197
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -