Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein

Xinhua Zhan, Charles Kim, Frank R Sharp

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.

Original languageEnglish (US)
Pages (from-to)183-197
Number of pages15
JournalBrain Research
Volume1234
DOIs
StatePublished - Oct 9 2008

Fingerprint

Transient Ischemic Attack
Microglia
Ischemia
In Situ Nick-End Labeling
Middle Cerebral Artery
Brain
Neurons
Proteins
Middle Cerebral Artery Infarction
Inclusion Bodies
Oligodendroglia
Brain Ischemia
Neuroglia
Caspase 3
Astrocytes
Sutures
Reperfusion
Macrophages

Keywords

  • Brain
  • Heat shock protein
  • Infarction
  • Ischemia
  • Microglia
  • Transient ischemic attacks (TIAs)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein. / Zhan, Xinhua; Kim, Charles; Sharp, Frank R.

In: Brain Research, Vol. 1234, 09.10.2008, p. 183-197.

Research output: Contribution to journalArticle

@article{7a08dd12678a451e81afbf96bf8b2b58,
title = "Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein",
abstract = "This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28{\%}) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated {"}bushy{"} microglia and Hsp70 negative, activated {"}polarized{"} or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.",
keywords = "Brain, Heat shock protein, Infarction, Ischemia, Microglia, Transient ischemic attacks (TIAs)",
author = "Xinhua Zhan and Charles Kim and Sharp, {Frank R}",
year = "2008",
month = "10",
day = "9",
doi = "10.1016/j.brainres.2008.07.094",
language = "English (US)",
volume = "1234",
pages = "183--197",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

TY - JOUR

T1 - Very brief focal ischemia simulating transient ischemic attacks (TIAs) can injure brain and induce Hsp70 protein

AU - Zhan, Xinhua

AU - Kim, Charles

AU - Sharp, Frank R

PY - 2008/10/9

Y1 - 2008/10/9

N2 - This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.

AB - This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throughout the entire MCA territory in many but not all animals. Following 5- and 10-minute MCA occlusions, 9 of 32 animals (28%) had microinfarcts mostly in dorsal lateral striatum. Uncommon Hsp70 stained intracellular cytoplasmic inclusions, some of which co-localized with activated caspase-3, were detected in microglia, macrophages, astrocytes and oligodendrocytes. Hsp70 stained neurons were TUNEL negative at 24 h and 48 h whereas some Hsp70 stained neurons were TUNEL positive at 72 h after reperfusion. Hsp70 positive, activated "bushy" microglia and Hsp70 negative, activated "polarized" or rod-shaped microglia were located outside of the microinfarcts. Thus, experimental focal ischemia simulating TIAs can: induce Hsp70 protein throughout the ischemic vessel territory; produce Hsp70 protein positive glial inclusions; activate Hsp70 positive and negative microglia; and cause microinfarcts in some animals.

KW - Brain

KW - Heat shock protein

KW - Infarction

KW - Ischemia

KW - Microglia

KW - Transient ischemic attacks (TIAs)

UR - http://www.scopus.com/inward/record.url?scp=52049112072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049112072&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2008.07.094

DO - 10.1016/j.brainres.2008.07.094

M3 - Article

C2 - 18708034

AN - SCOPUS:52049112072

VL - 1234

SP - 183

EP - 197

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -