Vertical Transmission of West Nile Virus by Three California Culex (Diptera: Culicidae) Species

Laura B. Goddard, Amy E. Roth, William Reisen, Thomas W. Scott

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Three California Culex species previously identified as efficient laboratory vectors of West Nile (WN) virus were tested for their capability to vertically transmit WN virus. Wild-caught Culex pipiens pipiens L., Culex pipiens quinquefasciatus Say, and two populations of Culex tarsalis Coquillett females were inoculated intrathoracically with 102.7 ± 0.1 plaque-forming units of WN virus. F1 progeny were reared at 18°C and subsequently tested as adults for infectious virus on Vero cell culture. Virus was not detected in 197 pools comprising 4,884 Cx. p. pipiens. The minimum filial infection rate (MFIR) for Cx. p. quinquefasciatus was ≈3.0/1,000 for 665 progeny tested in 28 pools. There was no virus detected in 102 pools of 2,453 progeny from Cx. tarsalis collected in Riverside County. The MFIR for Cx. tarsalis collected in Yolo County was ≈6.9/1,000 for 2,165 progeny tested in 86 pools. Mosquito progeny infected vertically during the fall could potentially serve as a mechanism for WN virus to overwinter and initiate horizontal transmission the following spring.

Original languageEnglish (US)
Pages (from-to)743-746
Number of pages4
JournalJournal of Medical Entomology
Volume40
Issue number6
DOIs
StatePublished - Jan 1 2003

Fingerprint

West Nile virus
Culex
Culicidae
Diptera
Viruses
viruses
Culex pipiens pipiens
Culex tarsalis
Vero Cells
Culex quinquefasciatus
Infection
infection
cell culture
Cell Culture Techniques
Population

Keywords

  • Culex pipiens
  • Culex tarsalis
  • Vertical transmission
  • West Nile virus

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Vertical Transmission of West Nile Virus by Three California Culex (Diptera : Culicidae) Species. / Goddard, Laura B.; Roth, Amy E.; Reisen, William; Scott, Thomas W.

In: Journal of Medical Entomology, Vol. 40, No. 6, 01.01.2003, p. 743-746.

Research output: Contribution to journalArticle

Goddard, Laura B. ; Roth, Amy E. ; Reisen, William ; Scott, Thomas W. / Vertical Transmission of West Nile Virus by Three California Culex (Diptera : Culicidae) Species. In: Journal of Medical Entomology. 2003 ; Vol. 40, No. 6. pp. 743-746.
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