Vertical transmission of TnSNPV, TnCPV, AcMNPV, and possibly recombinant NPV in Trichoplusia ni

J. R. Fuxa, A. R. Richter, A. O. Ameen, B. D. Hammock

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Four viruses were tested for vertical transmission in Trichoplusia ni: T. ni nucleopolyhedrovirus (TnSNPV), T. ni cypovirus (TnCPV), Autographa californica nucleopolyhedrovirus (AcMNPV), and AcMNPV engineered to express a scorpion toxin (AcMNPV.AaIT). Fifth instars were exposed to each virus, the survivors were reared and mated, and second-generation (F1) insects were examined for infection. TnSNPV was transmitted to offspring at a prevalence rate of 15.4%, TnCPV at 10.2%, and AcMNPV at 10.1%. Only one of 2484 F1 insects was infected with AcMNPV.AaIT; this experiment was repeated, and none of 4774 insects was infected. Thus, vertical transmission is unlikely to contribute to AcMNPV.AaIT contacting non-target organisms after its field release. There was evidence that TnCPV and possibly TnSNPV were activated to overt infections by ingestion of a different virus. TnCPV, but not the NPVs, routinely infected 0.3-1.7% of non-treated insects, probably indicating that it is vertically transmitted at enzootic levels.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalJournal of Invertebrate Pathology
Volume79
Issue number1
DOIs
StatePublished - 2002

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Keywords

  • Autographa californica NPV
  • Cypovirus
  • Nucleopolyhedrovirus
  • Recombinant NPV
  • Risk assessment
  • Transmission, vertical
  • Transmission, viral
  • Trichoplusia ni
  • Trichoplusia ni CPV
  • Trichoplusia ni NPV

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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