Vertebrate host susceptibility to Heartland Virus

Angela M. Bosco-Lauth, Amanda E. Calvert, J. Jeffrey Root, Tom Gidlewski, Brian H. Bird, Richard A. Bowen, Atis Muehlenbachs, Sherif R. Zaki, Aaron Brault

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Heartland virus (HRTV) is a recently described phlebovirus initially isolated in 2009 from 2 humans who had leukopenia and thrombocytopenia. Serologic assessment of domestic and wild animal populations near the residence of 1 of these persons showed high exposure rates to raccoons, white-tailed deer, and horses. To our knowledge, no laboratory-based assessments of viremic potential of animals infected with HRTV have been performed. We experimentally inoculated several vertebrates (raccoons, goats, chickens, rabbits, hamsters, C57BL/6 mice, and interferon-α/β/γ receptor–deficient [Ag129]) mice with this virus. All animals showed immune responses against HRTV after primary or secondary exposure. However, neutralizing antibody responses were limited. Only Ag129 mice showed detectable viremia and associated illness and death, which were dose dependent. Ag129 mice also showed development of mean peak viral antibody titers >8 log10 PFU/mL, hemorrhagic hepatic lesions, splenomegaly, and large amounts of HRTV antigen in mononuclear cells and hematopoietic cells in the spleen.

Original languageEnglish (US)
Pages (from-to)2070-2077
Number of pages8
JournalEmerging Infectious Diseases
Volume22
Issue number12
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

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    Bosco-Lauth, A. M., Calvert, A. E., Root, J. J., Gidlewski, T., Bird, B. H., Bowen, R. A., Muehlenbachs, A., Zaki, S. R., & Brault, A. (2016). Vertebrate host susceptibility to Heartland Virus. Emerging Infectious Diseases, 22(12), 2070-2077. https://doi.org/10.3201/eid2212.160472