Vein tissue expression of matrix metalloproteinase as biomarker for hemodialysis arteriovenous fistula maturation.

Eugene S Lee, Qiang Shen, Robert L. Pitts, Mingzhang Guo, Mack H. Wu, Sarah Y. Yuan

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Failure of arteriovenous fistula (AVF) maturation is attributed to impaired vein remodeling. The purpose of this study is to identify whether vein matrix metalloproteinase (MMP) expression and activity is associated with AVF maturation. Patients with renal insufficiency undergoing surgery had their vein segments harvested and snap-frozen at time of AVF construction. Expression of MMP-2, MMP-9, membrane type-1 MMP (MT1-MMP), tissue inhibitor of metallopreoteinases type 2 (TIMP-2), and TIMP-4 were measured using zymography and Western blotting techniques. Of 14 patients enrolled, 9 had successful maturation and 5 had failure of AVF maturation. Significantly higher levels of MT1-MMP (an MMP-2 activator; P = .01), TIMP-2 (an MMP-2 inhibitor; P = .03), MMP-2 latent (P = .02), and MMP-2 total (P = .03) were associated with AVF maturation. There was a trend toward higher levels of TIMP-4 in the successful group (P = .18). These data demonstrate a positive relationship between MMP-2 expression in veins and AVF maturation. MMP-2 could serve as a potential preoperative marker to predict maturation.

Original languageEnglish (US)
Pages (from-to)674-679
Number of pages6
JournalVascular and Endovascular Surgery
Volume44
Issue number8
StatePublished - Nov 2010

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

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