Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae

A. Wright, K. Maundrell, W. D. Heyer, D. Beach, P. Nurse

Research output: Contribution to journalArticle

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Abstract

We have constructed a variety of vectors for use in both budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe). Four of these, pDB262, pWH4, pWH5, and pMAK262, have positive selection for the insertion of cloned DNA, making them convenient for the construction of gene banks. pDB262, pWH4 and pWH5 contain the 2μ ARS and the LEU2 gene from S. cerevisiae and can be used for gene isolation. They can also be converted into integration vectors for use in the genetic mapping of cloned sequences. pMAK262 contains only the LEU2 gene from budding yeast and can be used to screen for ARS elements or for gene integration. We also describe two other integration vectors, pDAM3 and pDAM6, which have a variety of restriction sites suitable for subcloning.

Original languageEnglish (US)
Pages (from-to)156-158
Number of pages3
JournalPlasmid
Volume15
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology

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