V(D)J recombinase activity in a subset of germinal center B lymphocytes

Shuhua Han, Stacey R. Dillon, Biao Zheng, Michiko Shimoda, Mark S. Schlissel, Garnett Kelsoe

Research output: Contribution to journalArticlepeer-review

247 Scopus citations

Abstract

Reexpression of the V(D)J recombinase-activating genes RAG1 and RAG2 in germinal center B cells creates the potential for immunoglobulin gene rearrangement and the generation of new antigen receptor specificities. Intermediate products of V(D)J recombination are abundant in a subset of germinal center B Cells, demonstrating that the K immunoglobulin light-chain locus becomes a substrate for renewed V(D)J recombinase activity. This recombinationally active cell compartment contains many heavy-chain VDJ rearrangements that encode low-affinity or nonfunctional antibody. In germinal centers, secondary V(D)J recombination may be induced by diminished binding to antigen ligands, thereby limiting abrupt changes in receptor specificity to B cells that are usually eliminated from the germinal center reaction. This restriction preserves efficient antigen-driven selection in germinal centers while allowing for saltations in the somatic evolution of B cells.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalScience
Volume278
Issue number5336
DOIs
StatePublished - Oct 10 1997
Externally publishedYes

ASJC Scopus subject areas

  • General

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