V(D)J recombinase activity in a subset of germinal center B lymphocytes

Shuhua Han, Stacey R. Dillon, Biao Zheng, Michiko Shimoda, Mark S. Schlissel, Garnett Kelsoe

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

Reexpression of the V(D)J recombinase-activating genes RAG1 and RAG2 in germinal center B cells creates the potential for immunoglobulin gene rearrangement and the generation of new antigen receptor specificities. Intermediate products of V(D)J recombination are abundant in a subset of germinal center B Cells, demonstrating that the K immunoglobulin light-chain locus becomes a substrate for renewed V(D)J recombinase activity. This recombinationally active cell compartment contains many heavy-chain VDJ rearrangements that encode low-affinity or nonfunctional antibody. In germinal centers, secondary V(D)J recombination may be induced by diminished binding to antigen ligands, thereby limiting abrupt changes in receptor specificity to B cells that are usually eliminated from the germinal center reaction. This restriction preserves efficient antigen-driven selection in germinal centers while allowing for saltations in the somatic evolution of B cells.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalScience
Volume278
Issue number5336
DOIs
StatePublished - Oct 10 1997
Externally publishedYes

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VDJ Recombinases
Germinal Center
B-Lymphocytes
V(D)J Recombination
Clonal Evolution
Immunoglobulin Light Chains
Antigens
Immunoglobulin Genes
Antigen Receptors
Gene Rearrangement
Ligands
Antibodies
Genes

ASJC Scopus subject areas

  • General

Cite this

Han, S., Dillon, S. R., Zheng, B., Shimoda, M., Schlissel, M. S., & Kelsoe, G. (1997). V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science, 278(5336), 301-305. https://doi.org/10.1126/science.278.5336.301

V(D)J recombinase activity in a subset of germinal center B lymphocytes. / Han, Shuhua; Dillon, Stacey R.; Zheng, Biao; Shimoda, Michiko; Schlissel, Mark S.; Kelsoe, Garnett.

In: Science, Vol. 278, No. 5336, 10.10.1997, p. 301-305.

Research output: Contribution to journalArticle

Han, S, Dillon, SR, Zheng, B, Shimoda, M, Schlissel, MS & Kelsoe, G 1997, 'V(D)J recombinase activity in a subset of germinal center B lymphocytes', Science, vol. 278, no. 5336, pp. 301-305. https://doi.org/10.1126/science.278.5336.301
Han, Shuhua ; Dillon, Stacey R. ; Zheng, Biao ; Shimoda, Michiko ; Schlissel, Mark S. ; Kelsoe, Garnett. / V(D)J recombinase activity in a subset of germinal center B lymphocytes. In: Science. 1997 ; Vol. 278, No. 5336. pp. 301-305.
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