Abstract
Reexpression of the V(D)J recombinase-activating genes RAG1 and RAG2 in germinal center B cells creates the potential for immunoglobulin gene rearrangement and the generation of new antigen receptor specificities. Intermediate products of V(D)J recombination are abundant in a subset of germinal center B Cells, demonstrating that the K immunoglobulin light-chain locus becomes a substrate for renewed V(D)J recombinase activity. This recombinationally active cell compartment contains many heavy-chain VDJ rearrangements that encode low-affinity or nonfunctional antibody. In germinal centers, secondary V(D)J recombination may be induced by diminished binding to antigen ligands, thereby limiting abrupt changes in receptor specificity to B cells that are usually eliminated from the germinal center reaction. This restriction preserves efficient antigen-driven selection in germinal centers while allowing for saltations in the somatic evolution of B cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 301-305 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 278 |
| Issue number | 5336 |
| DOIs | |
| State | Published - Oct 10 1997 |
| Externally published | Yes |
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Cite this
V(D)J recombinase activity in a subset of germinal center B lymphocytes. / Han, Shuhua; Dillon, Stacey R.; Zheng, Biao; Shimoda, Michiko; Schlissel, Mark S.; Kelsoe, Garnett.
In: Science, Vol. 278, No. 5336, 10.10.1997, p. 301-305.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - V(D)J recombinase activity in a subset of germinal center B lymphocytes
AU - Han, Shuhua
AU - Dillon, Stacey R.
AU - Zheng, Biao
AU - Shimoda, Michiko
AU - Schlissel, Mark S.
AU - Kelsoe, Garnett
PY - 1997/10/10
Y1 - 1997/10/10
N2 - Reexpression of the V(D)J recombinase-activating genes RAG1 and RAG2 in germinal center B cells creates the potential for immunoglobulin gene rearrangement and the generation of new antigen receptor specificities. Intermediate products of V(D)J recombination are abundant in a subset of germinal center B Cells, demonstrating that the K immunoglobulin light-chain locus becomes a substrate for renewed V(D)J recombinase activity. This recombinationally active cell compartment contains many heavy-chain VDJ rearrangements that encode low-affinity or nonfunctional antibody. In germinal centers, secondary V(D)J recombination may be induced by diminished binding to antigen ligands, thereby limiting abrupt changes in receptor specificity to B cells that are usually eliminated from the germinal center reaction. This restriction preserves efficient antigen-driven selection in germinal centers while allowing for saltations in the somatic evolution of B cells.
AB - Reexpression of the V(D)J recombinase-activating genes RAG1 and RAG2 in germinal center B cells creates the potential for immunoglobulin gene rearrangement and the generation of new antigen receptor specificities. Intermediate products of V(D)J recombination are abundant in a subset of germinal center B Cells, demonstrating that the K immunoglobulin light-chain locus becomes a substrate for renewed V(D)J recombinase activity. This recombinationally active cell compartment contains many heavy-chain VDJ rearrangements that encode low-affinity or nonfunctional antibody. In germinal centers, secondary V(D)J recombination may be induced by diminished binding to antigen ligands, thereby limiting abrupt changes in receptor specificity to B cells that are usually eliminated from the germinal center reaction. This restriction preserves efficient antigen-driven selection in germinal centers while allowing for saltations in the somatic evolution of B cells.
UR - http://www.scopus.com/inward/record.url?scp=0030764978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030764978&partnerID=8YFLogxK
U2 - 10.1126/science.278.5336.301
DO - 10.1126/science.278.5336.301
M3 - Article
C2 - 9323211
AN - SCOPUS:0030764978
VL - 278
SP - 301
EP - 305
JO - Science
JF - Science
SN - 0036-8075
IS - 5336
ER -