Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts

Cedric G. Francois, Pascal C R Brouha, Luis A. Laurentin-Perez, Gustavo Perez-Abadia, Federico V. Grossi, John H. Barker, Charles W. Hewitt, Moshe Kon, Rajendra Ramsamooj, Claudio Maldonado

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BACKGROUND. The role of lymph nodes (LNs) in adaptive immune responses has been the subject of extensive research. In previous studies, the surgical removal of lymph nodes from rat hind limbs prevented the development of lethal graft-versus-host disease (GVHD) after allogeneic hind limb transplantation to chimeric recipient rats. The purpose of this study was to establish the role of the cellular fraction versus the microenvironment of LNs in the development of GVHD in this model. METHODS. A rat model for vascularized LN transplantation was developed and graft-versus-host responses were compared after: 1) naive ACI LN cells were infused into Wistar-Furth (WF) rats as chimeric recipients (e.g. [ACI→WF]); 2) vascularized WF lymph nodes were transplanted to syngeneic WF recipients; 3) nonvascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients; 4) vascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients. RESULTS. Transplantation of vascularized ACI lymph nodes to [ACI→WF] chimeric recipient rats resulted in severe and sometimes lethal GVHD. In contrast, neither the infusion of purified ACI LN cells nor the transplantation of nonvascularized LNs led to GVHD in chimeric recipients. CONCLUSIONS. When introducing allogeneic cells into chimeric recipients, concomitant transplantation of the vascularized LN microenvironment makes a manifest difference between induction and absence of GVHD. This illustrates the important role of the LN microenvironment in adaptive immune responses.

Original languageEnglish (US)
Pages (from-to)1435-1441
Number of pages7
JournalTransplantation
Volume81
Issue number10
DOIs
StatePublished - May 2006
Externally publishedYes

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Graft vs Host Disease
Transplantation
Lymph Nodes
Adaptive Immunity
Extremities
Inbred WF Rats
Cell Transplantation

Keywords

  • Graft-versus-host disease
  • T Lymphocytes
  • Tumor immunity

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Francois, C. G., Brouha, P. C. R., Laurentin-Perez, L. A., Perez-Abadia, G., Grossi, F. V., Barker, J. H., ... Maldonado, C. (2006). Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts. Transplantation, 81(10), 1435-1441. https://doi.org/10.1097/01.tp.0000209210.75211.57

Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts. / Francois, Cedric G.; Brouha, Pascal C R; Laurentin-Perez, Luis A.; Perez-Abadia, Gustavo; Grossi, Federico V.; Barker, John H.; Hewitt, Charles W.; Kon, Moshe; Ramsamooj, Rajendra; Maldonado, Claudio.

In: Transplantation, Vol. 81, No. 10, 05.2006, p. 1435-1441.

Research output: Contribution to journalArticle

Francois, CG, Brouha, PCR, Laurentin-Perez, LA, Perez-Abadia, G, Grossi, FV, Barker, JH, Hewitt, CW, Kon, M, Ramsamooj, R & Maldonado, C 2006, 'Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts', Transplantation, vol. 81, no. 10, pp. 1435-1441. https://doi.org/10.1097/01.tp.0000209210.75211.57
Francois CG, Brouha PCR, Laurentin-Perez LA, Perez-Abadia G, Grossi FV, Barker JH et al. Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts. Transplantation. 2006 May;81(10):1435-1441. https://doi.org/10.1097/01.tp.0000209210.75211.57
Francois, Cedric G. ; Brouha, Pascal C R ; Laurentin-Perez, Luis A. ; Perez-Abadia, Gustavo ; Grossi, Federico V. ; Barker, John H. ; Hewitt, Charles W. ; Kon, Moshe ; Ramsamooj, Rajendra ; Maldonado, Claudio. / Vascularized lymph node transplantation induces graft-versus-host disease in chimeric hosts. In: Transplantation. 2006 ; Vol. 81, No. 10. pp. 1435-1441.
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abstract = "BACKGROUND. The role of lymph nodes (LNs) in adaptive immune responses has been the subject of extensive research. In previous studies, the surgical removal of lymph nodes from rat hind limbs prevented the development of lethal graft-versus-host disease (GVHD) after allogeneic hind limb transplantation to chimeric recipient rats. The purpose of this study was to establish the role of the cellular fraction versus the microenvironment of LNs in the development of GVHD in this model. METHODS. A rat model for vascularized LN transplantation was developed and graft-versus-host responses were compared after: 1) naive ACI LN cells were infused into Wistar-Furth (WF) rats as chimeric recipients (e.g. [ACI→WF]); 2) vascularized WF lymph nodes were transplanted to syngeneic WF recipients; 3) nonvascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients; 4) vascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients. RESULTS. Transplantation of vascularized ACI lymph nodes to [ACI→WF] chimeric recipient rats resulted in severe and sometimes lethal GVHD. In contrast, neither the infusion of purified ACI LN cells nor the transplantation of nonvascularized LNs led to GVHD in chimeric recipients. CONCLUSIONS. When introducing allogeneic cells into chimeric recipients, concomitant transplantation of the vascularized LN microenvironment makes a manifest difference between induction and absence of GVHD. This illustrates the important role of the LN microenvironment in adaptive immune responses.",
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AU - Laurentin-Perez, Luis A.

AU - Perez-Abadia, Gustavo

AU - Grossi, Federico V.

AU - Barker, John H.

AU - Hewitt, Charles W.

AU - Kon, Moshe

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AU - Maldonado, Claudio

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N2 - BACKGROUND. The role of lymph nodes (LNs) in adaptive immune responses has been the subject of extensive research. In previous studies, the surgical removal of lymph nodes from rat hind limbs prevented the development of lethal graft-versus-host disease (GVHD) after allogeneic hind limb transplantation to chimeric recipient rats. The purpose of this study was to establish the role of the cellular fraction versus the microenvironment of LNs in the development of GVHD in this model. METHODS. A rat model for vascularized LN transplantation was developed and graft-versus-host responses were compared after: 1) naive ACI LN cells were infused into Wistar-Furth (WF) rats as chimeric recipients (e.g. [ACI→WF]); 2) vascularized WF lymph nodes were transplanted to syngeneic WF recipients; 3) nonvascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients; 4) vascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients. RESULTS. Transplantation of vascularized ACI lymph nodes to [ACI→WF] chimeric recipient rats resulted in severe and sometimes lethal GVHD. In contrast, neither the infusion of purified ACI LN cells nor the transplantation of nonvascularized LNs led to GVHD in chimeric recipients. CONCLUSIONS. When introducing allogeneic cells into chimeric recipients, concomitant transplantation of the vascularized LN microenvironment makes a manifest difference between induction and absence of GVHD. This illustrates the important role of the LN microenvironment in adaptive immune responses.

AB - BACKGROUND. The role of lymph nodes (LNs) in adaptive immune responses has been the subject of extensive research. In previous studies, the surgical removal of lymph nodes from rat hind limbs prevented the development of lethal graft-versus-host disease (GVHD) after allogeneic hind limb transplantation to chimeric recipient rats. The purpose of this study was to establish the role of the cellular fraction versus the microenvironment of LNs in the development of GVHD in this model. METHODS. A rat model for vascularized LN transplantation was developed and graft-versus-host responses were compared after: 1) naive ACI LN cells were infused into Wistar-Furth (WF) rats as chimeric recipients (e.g. [ACI→WF]); 2) vascularized WF lymph nodes were transplanted to syngeneic WF recipients; 3) nonvascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients; 4) vascularized ACI lymph nodes were transplanted to [ACI→WF] chimeric recipients. RESULTS. Transplantation of vascularized ACI lymph nodes to [ACI→WF] chimeric recipient rats resulted in severe and sometimes lethal GVHD. In contrast, neither the infusion of purified ACI LN cells nor the transplantation of nonvascularized LNs led to GVHD in chimeric recipients. CONCLUSIONS. When introducing allogeneic cells into chimeric recipients, concomitant transplantation of the vascularized LN microenvironment makes a manifest difference between induction and absence of GVHD. This illustrates the important role of the LN microenvironment in adaptive immune responses.

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