Vascular invasion of cartilage: Correlation of morphology with lysozyme, glycosaminoglycans, protease, and protease-inhibitory activity during endochondral bone development

A Hari Reddi, Klaus E. Kuettner

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32 Citations (Scopus)

Abstract

Changes in the levels of lysozyme, patterns of glycosaminoglycans, and activities of proteases and protease inhibitors were studied during matrix-induced cartilage, bone, and bone marrow development. The morphological transitions were correlated with the biochemical parameters. There was a peak in lysozyme content on Day 3, during mesenchymal cell proliferation, followed by a decline during endochondral bone formation. The lysozyme levels increased again and attained maximal values during hematopoiesis on Day 21. Protease-inhibitory activity was maximal during chondrogenesis and diminished during osteogenesis. Protease activity was maximal on Day 3 during mesenchymal cell proliferation and was apparently present as an enzyme-inhibitor complex. Vascularization and bone formation were accompanied by an increase in protease activity. Chondroitin-4-sulfate was the predominant glycosaminoglycan detected in the matrix-induced cartilage and bone.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalDevelopmental Biology
Volume82
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

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Bone Development
Muramidase
Glycosaminoglycans
Cartilage
Blood Vessels
Peptide Hydrolases
Osteogenesis
Cell Proliferation
Chondrogenesis
Bone and Bones
Chondroitin Sulfates
Hematopoiesis
Enzyme Inhibitors
Protease Inhibitors
Bone Marrow

ASJC Scopus subject areas

  • Developmental Biology

Cite this

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abstract = "Changes in the levels of lysozyme, patterns of glycosaminoglycans, and activities of proteases and protease inhibitors were studied during matrix-induced cartilage, bone, and bone marrow development. The morphological transitions were correlated with the biochemical parameters. There was a peak in lysozyme content on Day 3, during mesenchymal cell proliferation, followed by a decline during endochondral bone formation. The lysozyme levels increased again and attained maximal values during hematopoiesis on Day 21. Protease-inhibitory activity was maximal during chondrogenesis and diminished during osteogenesis. Protease activity was maximal on Day 3 during mesenchymal cell proliferation and was apparently present as an enzyme-inhibitor complex. Vascularization and bone formation were accompanied by an increase in protease activity. Chondroitin-4-sulfate was the predominant glycosaminoglycan detected in the matrix-induced cartilage and bone.",
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T2 - Correlation of morphology with lysozyme, glycosaminoglycans, protease, and protease-inhibitory activity during endochondral bone development

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AU - Kuettner, Klaus E.

PY - 1981

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