Abstract
Vascular endothelial growth factor (VEGF) is an important regulator of tumor angiogenesis and vascular permeability, and has been implicated both in progression of central nervous system (CNS) tumors and development of vasogenic peritumoral edema. A retrospective study was done to characterize the levels of expression of the 3 major canine VEGF isoforms (VEGF120, VEGF 164, VEGF188) in a variety of spontaneous canine CNS tumors using quantitative TaqMan reverse transcription real-time polymerase chain reaction. Presence and degree of peritumoral edema also were determined in sampled tumors using magnetic resonance imaging (MRI). Increased expression of VEGF relative to normal cerebral cortex tissue was seen predominantly in high grade astrocytic (grade IV) and oligodendroglial (grade III) tumors, with lower expression in low grade astrocytomas (grade II) and meningiomas (grade I). All 3 major VEGF isoforms were present; VEGF164 was the predominant isoform, particularly in the tumors with the highest VEGF expression. Peritumoral edema was present in all tumor types; however, a significant association between the extent of peritumoral edema and the level of VEGF expression was not apparent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 131-139 |
| Number of pages | 9 |
| Journal | Veterinary Pathology |
| Volume | 45 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 2008 |
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Keywords
- Canine
- Central nervous system tumors
- Edema
- Reverse transcriptase polymerase chain reaction
- Vascular endothelial growth factor
- VEGF
ASJC Scopus subject areas
- veterinary(all)
Cite this
Vascular endothelial growth factor mRNA expression and peritumoral edema in canine primary central nervous system tumors. / Dickinson, Peter J; Sturges, Beverly; Higgins, Robert; Roberts, B. N.; Leutenegger, C. M.; Bollen, A. W.; Lecouteur, Richard A.
In: Veterinary Pathology, Vol. 45, No. 2, 03.2008, p. 131-139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular endothelial growth factor mRNA expression and peritumoral edema in canine primary central nervous system tumors
AU - Dickinson, Peter J
AU - Sturges, Beverly
AU - Higgins, Robert
AU - Roberts, B. N.
AU - Leutenegger, C. M.
AU - Bollen, A. W.
AU - Lecouteur, Richard A
PY - 2008/3
Y1 - 2008/3
N2 - Vascular endothelial growth factor (VEGF) is an important regulator of tumor angiogenesis and vascular permeability, and has been implicated both in progression of central nervous system (CNS) tumors and development of vasogenic peritumoral edema. A retrospective study was done to characterize the levels of expression of the 3 major canine VEGF isoforms (VEGF120, VEGF 164, VEGF188) in a variety of spontaneous canine CNS tumors using quantitative TaqMan reverse transcription real-time polymerase chain reaction. Presence and degree of peritumoral edema also were determined in sampled tumors using magnetic resonance imaging (MRI). Increased expression of VEGF relative to normal cerebral cortex tissue was seen predominantly in high grade astrocytic (grade IV) and oligodendroglial (grade III) tumors, with lower expression in low grade astrocytomas (grade II) and meningiomas (grade I). All 3 major VEGF isoforms were present; VEGF164 was the predominant isoform, particularly in the tumors with the highest VEGF expression. Peritumoral edema was present in all tumor types; however, a significant association between the extent of peritumoral edema and the level of VEGF expression was not apparent.
AB - Vascular endothelial growth factor (VEGF) is an important regulator of tumor angiogenesis and vascular permeability, and has been implicated both in progression of central nervous system (CNS) tumors and development of vasogenic peritumoral edema. A retrospective study was done to characterize the levels of expression of the 3 major canine VEGF isoforms (VEGF120, VEGF 164, VEGF188) in a variety of spontaneous canine CNS tumors using quantitative TaqMan reverse transcription real-time polymerase chain reaction. Presence and degree of peritumoral edema also were determined in sampled tumors using magnetic resonance imaging (MRI). Increased expression of VEGF relative to normal cerebral cortex tissue was seen predominantly in high grade astrocytic (grade IV) and oligodendroglial (grade III) tumors, with lower expression in low grade astrocytomas (grade II) and meningiomas (grade I). All 3 major VEGF isoforms were present; VEGF164 was the predominant isoform, particularly in the tumors with the highest VEGF expression. Peritumoral edema was present in all tumor types; however, a significant association between the extent of peritumoral edema and the level of VEGF expression was not apparent.
KW - Canine
KW - Central nervous system tumors
KW - Edema
KW - Reverse transcriptase polymerase chain reaction
KW - Vascular endothelial growth factor
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=42449139600&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42449139600&partnerID=8YFLogxK
U2 - 10.1354/vp.45-2-131
DO - 10.1354/vp.45-2-131
M3 - Article
C2 - 18424825
AN - SCOPUS:42449139600
VL - 45
SP - 131
EP - 139
JO - Veterinary Pathology
JF - Veterinary Pathology
SN - 0300-9858
IS - 2
ER -