Abstract
These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Heart and Circulatory Physiology |
| Volume | 271 |
| Issue number | 6 40-6 |
| State | Published - 1996 |
Fingerprint
Keywords
- capillary permeability
- frog
- heterogeneity
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels. / Bates, D. O.; Curry, F. E.
In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 271, No. 6 40-6, 1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels
AU - Bates, D. O.
AU - Curry, F. E.
PY - 1996
Y1 - 1996
N2 - These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.
AB - These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.
KW - capillary permeability
KW - frog
KW - heterogeneity
UR - http://www.scopus.com/inward/record.url?scp=0030457695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030457695&partnerID=8YFLogxK
M3 - Article
C2 - 8997313
AN - SCOPUS:0030457695
VL - 271
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 6 40-6
ER -