Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels

D. O. Bates, F. E. Curry

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume271
Issue number6 40-6
StatePublished - 1996

Fingerprint

Microvessels
Vascular Endothelial Growth Factor A
Mesentery
Capillary Permeability
Anura
Permeability
Perfusion
Water

Keywords

  • capillary permeability
  • frog
  • heterogeneity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels. / Bates, D. O.; Curry, F. E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 271, No. 6 40-6, 1996.

Research output: Contribution to journalArticle

@article{4ecf7279283b4979957e366f6772eb1c,
title = "Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels",
abstract = "These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.",
keywords = "capillary permeability, frog, heterogeneity",
author = "Bates, {D. O.} and Curry, {F. E.}",
year = "1996",
language = "English (US)",
volume = "271",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 40-6",

}

TY - JOUR

T1 - Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels

AU - Bates, D. O.

AU - Curry, F. E.

PY - 1996

Y1 - 1996

N2 - These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.

AB - These experiments report the first direct measurement of microvessel permeability coefficients after exposure to vascular endothelial growth factor (VEGF). The Landis technique was extended to enable measurement of the resistance of the microvessel wall to water flow, hydraulic conductivity (L(p)), on the same microvessel in the frog mesentery during the initial exposure to VEGF (acute) and 24 and 72 h after initial exposure (chronic). Control measurements of L(p) showed no change either acutely or chronically. Exposure to 1 nM VEGF rapidly and transiently increased microvessel L(p) within 30 s (to 7.8-fold greater than baseline values) and returned to control within 2 min. The baseline L(p) was fivefold greater after 24 h than the initial baseline as a result of VEGF perfusion and returned to its original value after 72 h. These experiments confirm the hypothesis that VEGF acts both acutely (over a period of a few minutes) and chronically (over a few hours) to increase microvascular permeability.

KW - capillary permeability

KW - frog

KW - heterogeneity

UR - http://www.scopus.com/inward/record.url?scp=0030457695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030457695&partnerID=8YFLogxK

M3 - Article

C2 - 8997313

AN - SCOPUS:0030457695

VL - 271

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 6 40-6

ER -