Variation and in vitro splicing of group I introns in rRNA genes of Pneumocystis carinii

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The sequences of the rRNA genes of Pneumocystis carinii from rat and human sources demonstrate three distinct genotypes based on the group I introns present in these genes. One rat isolate (Pc1) contains such introns in its 16S and 26S rRNA genes, while another rat isolate (Pc2) and a human isolate (Pc3) only contain an intron in the 26S rRNA gene. The four introns all catalyze their own excision from RNA transcripts, and this reaction is inhibited by the anti-pneumocystis drug pentamidine and its analogues. Although they differ in sequence, they are more similar to one another than to group I introns found in other eukaryotic microbes.

Original languageEnglish (US)
Pages (from-to)2415-2421
Number of pages7
JournalNucleic Acids Research
Volume21
Issue number10
StatePublished - May 25 1993
Externally publishedYes

Fingerprint

Pneumocystis carinii
rRNA Genes
Introns
Genes
Gene
Rats
Pneumocystis
Pentamidine
Genotype
RNA
Drugs
Analogue
Distinct
In Vitro Techniques
Demonstrate
Rat
Pharmaceutical Preparations
Human

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Variation and in vitro splicing of group I introns in rRNA genes of Pneumocystis carinii. / Liu, Yong; Leibowitz, Michael J.

In: Nucleic Acids Research, Vol. 21, No. 10, 25.05.1993, p. 2415-2421.

Research output: Contribution to journalArticle

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