Variation amongst the neutralizing epitopes of bluetongue viruses isolated in the United States in 1979-1981

Nigel J Maclachlan, P. V. Rossitto, H. W. Heidner, L. G. Iezzi, Tilahun Yilma, C. D. DeMaula, Bennie Osburn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Neutralizing epitopes present on field isolates of bluetongue virus (BTV) serotypes 10, 11, 13 and 17 were evaluated with a panel of polyclonal and neutralizing monoclonal antibodies (MAbs). A total of 91 field isolates were evaluated, including 15 isolates of BTV-10, 29 isolates of BTV-11, 26 isolates of BTV-13, and 21 isolates of BTV-17. The viruses were isolated from cattle, goats, sheep, elk and deer in Idaho, Louisiana, Nebraska and, predominantly, California, in the years 1979, 1980 and 1981. The isolates were analyzed and compared using a panel of neutralizing MAbs which included five MAbs raised against BTV-2, seven against BTV-10, five against BTV-13, and six against BTV-17. Neutralization patterns obtained with the MAb panel and individual field isolates were compared to those obtained with prototype viruses of each serotype. All field isolates were neutralized by at least some of the MAbs raised against the prototype virus of the same serotype. All field isolates of BTV-10 were neutralized by the seven MAbs raised to BTV-10, whereas the field isolates of BTV-11, BTV-13 and BTV-17 were not consistently neutralized by all of the MAbs raised against the prototype virus of the same serotype. Variation in neutralizing epitopes recognized by the MAb panel was most pronounced amongst the field isolates of BTV-17. A one-way cross neutralization was evident between BTV-10 and BTV-17 as all field isolats of BTV-17 were neutralized by four of the MAbs raised against BTV-10. In contrast, no BTV-10 isolates were neutralized by the MAbs raised against BTV-17. Differences in the MAb neutralization patterns of field isolates of BTV-11, BTV-13 and BTV-17 suggest that the immunogenic domain responsible for their neutralization is plastic, such that individual epitopes within the domain may vary in their significance to the neutralization of different viruses, even of the same serotype. The apparent conservation of neutralizing epitopes on field isolates of BTV-10 suggests that the field isolates may be derived from the modified-live vaccine strain of BTV-10.

Original languageEnglish (US)
Pages (from-to)303-316
Number of pages14
JournalVeterinary Microbiology
Volume31
Issue number4
DOIs
StatePublished - Jun 15 1992

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Bluetongue virus
neutralization
epitopes
Epitopes
monoclonal antibodies
Monoclonal Antibodies
serotypes
Viruses
prototypes
viruses
Neutralizing Antibodies
neutralizing antibodies

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Variation amongst the neutralizing epitopes of bluetongue viruses isolated in the United States in 1979-1981. / Maclachlan, Nigel J; Rossitto, P. V.; Heidner, H. W.; Iezzi, L. G.; Yilma, Tilahun; DeMaula, C. D.; Osburn, Bennie.

In: Veterinary Microbiology, Vol. 31, No. 4, 15.06.1992, p. 303-316.

Research output: Contribution to journalArticle

Maclachlan, Nigel J ; Rossitto, P. V. ; Heidner, H. W. ; Iezzi, L. G. ; Yilma, Tilahun ; DeMaula, C. D. ; Osburn, Bennie. / Variation amongst the neutralizing epitopes of bluetongue viruses isolated in the United States in 1979-1981. In: Veterinary Microbiology. 1992 ; Vol. 31, No. 4. pp. 303-316.
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AU - DeMaula, C. D.

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N2 - Neutralizing epitopes present on field isolates of bluetongue virus (BTV) serotypes 10, 11, 13 and 17 were evaluated with a panel of polyclonal and neutralizing monoclonal antibodies (MAbs). A total of 91 field isolates were evaluated, including 15 isolates of BTV-10, 29 isolates of BTV-11, 26 isolates of BTV-13, and 21 isolates of BTV-17. The viruses were isolated from cattle, goats, sheep, elk and deer in Idaho, Louisiana, Nebraska and, predominantly, California, in the years 1979, 1980 and 1981. The isolates were analyzed and compared using a panel of neutralizing MAbs which included five MAbs raised against BTV-2, seven against BTV-10, five against BTV-13, and six against BTV-17. Neutralization patterns obtained with the MAb panel and individual field isolates were compared to those obtained with prototype viruses of each serotype. All field isolates were neutralized by at least some of the MAbs raised against the prototype virus of the same serotype. All field isolates of BTV-10 were neutralized by the seven MAbs raised to BTV-10, whereas the field isolates of BTV-11, BTV-13 and BTV-17 were not consistently neutralized by all of the MAbs raised against the prototype virus of the same serotype. Variation in neutralizing epitopes recognized by the MAb panel was most pronounced amongst the field isolates of BTV-17. A one-way cross neutralization was evident between BTV-10 and BTV-17 as all field isolats of BTV-17 were neutralized by four of the MAbs raised against BTV-10. In contrast, no BTV-10 isolates were neutralized by the MAbs raised against BTV-17. Differences in the MAb neutralization patterns of field isolates of BTV-11, BTV-13 and BTV-17 suggest that the immunogenic domain responsible for their neutralization is plastic, such that individual epitopes within the domain may vary in their significance to the neutralization of different viruses, even of the same serotype. The apparent conservation of neutralizing epitopes on field isolates of BTV-10 suggests that the field isolates may be derived from the modified-live vaccine strain of BTV-10.

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