Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)

Samuel P. Franklin, Jennifer L. Troyer, Julie A. TerWee, Lisa M. Lyren, Roland W. Kays, Seth P D Riley, Walter M Boyce, Kevin R. Crooks, Sue Vandewoude

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although lentiviruses similar to feline immunodeficiency virus (FIV) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. We tested bobcats (Lynx rufus), pumas (Felis concolor), and ocelots (Leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (PLV), African lion lentivirus (LLV), and domestic cat FIV-based immunoblots, a commercially available enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR). Puma lentivirus immunoblots identified more seropositive individuals than the other antibody-detection assays. The commercial ELISA provided a fair ability to recognize seropositive samples when compared with PLV immunoblot for screening bobcats and ocelots, but not pumas. Polymerase chain reaction identified fewer positive samples than PLV immunoblot for all three species. Immunoblot results were equivalent whether the sample tested was serum, plasma, or whole blood. The results from this study and previous investigations suggest that the PLV immunoblot has the greatest ability to detect reactive samples when screening wild felids of North America and is unlikely to produce false positive results. However, the commercial ELISA kit may provide ap adequate alternative for screening of some species and is more easily adapted to field conditions.

Original languageEnglish (US)
Pages (from-to)700-710
Number of pages11
JournalJournal of Wildlife Diseases
Volume43
Issue number4
StatePublished - Oct 2007

Fingerprint

Puma lentivirus
Lynx
Puma
Leopardus pardalis
Feline Immunodeficiency Virus
Lynx rufus
Puma concolor
Felidae
Lentivirus
assay
assays
Infection
Feline immunodeficiency virus
infection
felid
enzyme-linked immunosorbent assay
screening
enzyme
Enzyme-Linked Immunosorbent Assay
polymerase chain reaction

Keywords

  • Bobcat
  • ELISA
  • FIV
  • Immunoblot
  • Lentivirus
  • Ocelot
  • PCR
  • Puma

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Franklin, S. P., Troyer, J. L., TerWee, J. A., Lyren, L. M., Kays, R. W., Riley, S. P. D., ... Vandewoude, S. (2007). Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis). Journal of Wildlife Diseases, 43(4), 700-710.

Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis). / Franklin, Samuel P.; Troyer, Jennifer L.; TerWee, Julie A.; Lyren, Lisa M.; Kays, Roland W.; Riley, Seth P D; Boyce, Walter M; Crooks, Kevin R.; Vandewoude, Sue.

In: Journal of Wildlife Diseases, Vol. 43, No. 4, 10.2007, p. 700-710.

Research output: Contribution to journalArticle

Franklin, SP, Troyer, JL, TerWee, JA, Lyren, LM, Kays, RW, Riley, SPD, Boyce, WM, Crooks, KR & Vandewoude, S 2007, 'Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)', Journal of Wildlife Diseases, vol. 43, no. 4, pp. 700-710.
Franklin, Samuel P. ; Troyer, Jennifer L. ; TerWee, Julie A. ; Lyren, Lisa M. ; Kays, Roland W. ; Riley, Seth P D ; Boyce, Walter M ; Crooks, Kevin R. ; Vandewoude, Sue. / Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis). In: Journal of Wildlife Diseases. 2007 ; Vol. 43, No. 4. pp. 700-710.
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abstract = "Although lentiviruses similar to feline immunodeficiency virus (FIV) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. We tested bobcats (Lynx rufus), pumas (Felis concolor), and ocelots (Leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (PLV), African lion lentivirus (LLV), and domestic cat FIV-based immunoblots, a commercially available enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR). Puma lentivirus immunoblots identified more seropositive individuals than the other antibody-detection assays. The commercial ELISA provided a fair ability to recognize seropositive samples when compared with PLV immunoblot for screening bobcats and ocelots, but not pumas. Polymerase chain reaction identified fewer positive samples than PLV immunoblot for all three species. Immunoblot results were equivalent whether the sample tested was serum, plasma, or whole blood. The results from this study and previous investigations suggest that the PLV immunoblot has the greatest ability to detect reactive samples when screening wild felids of North America and is unlikely to produce false positive results. However, the commercial ELISA kit may provide ap adequate alternative for screening of some species and is more easily adapted to field conditions.",
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