Vanadium treatment of diabetic Sprague-Dawley rats results in tissue vanadium accumulation and pro-oxidant effects

Michelle H. Oster, Juan M. Llobet, Jose L. Domingo, J. Bruce German, Carl L Keen

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The effect of sodium metavanadate (NaVO3) consumption on trace element metabolism, components of the antioxidant defense system and lipid oxidative damage were studied in control (CON) and streptozotocin-induced diabetic (DIAB) rats. Ten days after injection, CON and DIAB rats received either 0 mM NaVO3/80 mM NaCl (0 group) or 1.2 mM NaVO3/80 mM NaCl (1.2V group) in their drinking water. DIAB groups had higher food and fluid intakes than the CON groups; vanadium (V) groups had lower food and fluid intakes than the saline groups. Vanadium therapy lowered plasma glucose concentrations of DIAB rats. The following parameters were similar among the groups: plasma Zn, Cu and Fe concentrations, plasma ceruloplasmin activity, liver Zn, Cu, Mn and Fe concentrations, kidney Mn and Fe concentrations, liver non-Se-dependent glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Red) and Mn-SOD activities, liver reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations and kidney non-Se-dependent GSH-Px activity. Kidney Zn and Cu concentrations were higher in DIAB rats than in CON rats. The CON-1.2V and DIAB-1.2V groups had V accumulation in the liver and kidney. Liver CuZn-SOD and Se-dependent GSH-Px and kidney CuZn-SOD and GSH-Red activities were lower in DIAB rats compared to CON rats; kidney Mn-SOD and kidney Se-dependent GSH-Px activities were higher in DIAB rats than CON rats. Vanadium treatment did not cause significant alterations in the antioxidant defense system; however, tissue vandium concentrations were positively correlated to TBARS production. These results s3ow that diabetes caused significant alterations in the antioxidant defence system and that V therapy was associated with a marked deterioration in health of both control and diabetic rats.

Original languageEnglish (US)
Pages (from-to)115-130
Number of pages16
JournalToxicology
Volume83
Issue number1-3
DOIs
StatePublished - Oct 25 1993

Fingerprint

Vanadium
Sprague Dawley Rats
Rats
Reactive Oxygen Species
Tissue
Liver
Rat control
Kidney
Glutathione Disulfide
Glutathione Reductase
Antioxidants
Plasmas
Therapeutics
Superoxide Dismutase
Ceruloplasmin
Vanadates
Fluids
Trace Elements
Eating
Streptozocin

Keywords

  • Antioxidant defense
  • Diabetes
  • Lipid peroxidation
  • Trace elements
  • Vanadium

ASJC Scopus subject areas

  • Toxicology

Cite this

Vanadium treatment of diabetic Sprague-Dawley rats results in tissue vanadium accumulation and pro-oxidant effects. / Oster, Michelle H.; Llobet, Juan M.; Domingo, Jose L.; Bruce German, J.; Keen, Carl L.

In: Toxicology, Vol. 83, No. 1-3, 25.10.1993, p. 115-130.

Research output: Contribution to journalArticle

Oster, Michelle H. ; Llobet, Juan M. ; Domingo, Jose L. ; Bruce German, J. ; Keen, Carl L. / Vanadium treatment of diabetic Sprague-Dawley rats results in tissue vanadium accumulation and pro-oxidant effects. In: Toxicology. 1993 ; Vol. 83, No. 1-3. pp. 115-130.
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AB - The effect of sodium metavanadate (NaVO3) consumption on trace element metabolism, components of the antioxidant defense system and lipid oxidative damage were studied in control (CON) and streptozotocin-induced diabetic (DIAB) rats. Ten days after injection, CON and DIAB rats received either 0 mM NaVO3/80 mM NaCl (0 group) or 1.2 mM NaVO3/80 mM NaCl (1.2V group) in their drinking water. DIAB groups had higher food and fluid intakes than the CON groups; vanadium (V) groups had lower food and fluid intakes than the saline groups. Vanadium therapy lowered plasma glucose concentrations of DIAB rats. The following parameters were similar among the groups: plasma Zn, Cu and Fe concentrations, plasma ceruloplasmin activity, liver Zn, Cu, Mn and Fe concentrations, kidney Mn and Fe concentrations, liver non-Se-dependent glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Red) and Mn-SOD activities, liver reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations and kidney non-Se-dependent GSH-Px activity. Kidney Zn and Cu concentrations were higher in DIAB rats than in CON rats. The CON-1.2V and DIAB-1.2V groups had V accumulation in the liver and kidney. Liver CuZn-SOD and Se-dependent GSH-Px and kidney CuZn-SOD and GSH-Red activities were lower in DIAB rats compared to CON rats; kidney Mn-SOD and kidney Se-dependent GSH-Px activities were higher in DIAB rats than CON rats. Vanadium treatment did not cause significant alterations in the antioxidant defense system; however, tissue vandium concentrations were positively correlated to TBARS production. These results s3ow that diabetes caused significant alterations in the antioxidant defence system and that V therapy was associated with a marked deterioration in health of both control and diabetic rats.

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