Abstract
The effect of sodium metavanadate (NaVO3) consumption on trace element metabolism, components of the antioxidant defense system and lipid oxidative damage were studied in control (CON) and streptozotocin-induced diabetic (DIAB) rats. Ten days after injection, CON and DIAB rats received either 0 mM NaVO3/80 mM NaCl (0 group) or 1.2 mM NaVO3/80 mM NaCl (1.2V group) in their drinking water. DIAB groups had higher food and fluid intakes than the CON groups; vanadium (V) groups had lower food and fluid intakes than the saline groups. Vanadium therapy lowered plasma glucose concentrations of DIAB rats. The following parameters were similar among the groups: plasma Zn, Cu and Fe concentrations, plasma ceruloplasmin activity, liver Zn, Cu, Mn and Fe concentrations, kidney Mn and Fe concentrations, liver non-Se-dependent glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Red) and Mn-SOD activities, liver reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations and kidney non-Se-dependent GSH-Px activity. Kidney Zn and Cu concentrations were higher in DIAB rats than in CON rats. The CON-1.2V and DIAB-1.2V groups had V accumulation in the liver and kidney. Liver CuZn-SOD and Se-dependent GSH-Px and kidney CuZn-SOD and GSH-Red activities were lower in DIAB rats compared to CON rats; kidney Mn-SOD and kidney Se-dependent GSH-Px activities were higher in DIAB rats than CON rats. Vanadium treatment did not cause significant alterations in the antioxidant defense system; however, tissue vandium concentrations were positively correlated to TBARS production. These results s3ow that diabetes caused significant alterations in the antioxidant defence system and that V therapy was associated with a marked deterioration in health of both control and diabetic rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-130 |
| Number of pages | 16 |
| Journal | Toxicology |
| Volume | 83 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Oct 25 1993 |
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Keywords
- Antioxidant defense
- Diabetes
- Lipid peroxidation
- Trace elements
- Vanadium
ASJC Scopus subject areas
- Toxicology
Cite this
Vanadium treatment of diabetic Sprague-Dawley rats results in tissue vanadium accumulation and pro-oxidant effects. / Oster, Michelle H.; Llobet, Juan M.; Domingo, Jose L.; Bruce German, J.; Keen, Carl L.
In: Toxicology, Vol. 83, No. 1-3, 25.10.1993, p. 115-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vanadium treatment of diabetic Sprague-Dawley rats results in tissue vanadium accumulation and pro-oxidant effects
AU - Oster, Michelle H.
AU - Llobet, Juan M.
AU - Domingo, Jose L.
AU - Bruce German, J.
AU - Keen, Carl L
PY - 1993/10/25
Y1 - 1993/10/25
N2 - The effect of sodium metavanadate (NaVO3) consumption on trace element metabolism, components of the antioxidant defense system and lipid oxidative damage were studied in control (CON) and streptozotocin-induced diabetic (DIAB) rats. Ten days after injection, CON and DIAB rats received either 0 mM NaVO3/80 mM NaCl (0 group) or 1.2 mM NaVO3/80 mM NaCl (1.2V group) in their drinking water. DIAB groups had higher food and fluid intakes than the CON groups; vanadium (V) groups had lower food and fluid intakes than the saline groups. Vanadium therapy lowered plasma glucose concentrations of DIAB rats. The following parameters were similar among the groups: plasma Zn, Cu and Fe concentrations, plasma ceruloplasmin activity, liver Zn, Cu, Mn and Fe concentrations, kidney Mn and Fe concentrations, liver non-Se-dependent glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Red) and Mn-SOD activities, liver reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations and kidney non-Se-dependent GSH-Px activity. Kidney Zn and Cu concentrations were higher in DIAB rats than in CON rats. The CON-1.2V and DIAB-1.2V groups had V accumulation in the liver and kidney. Liver CuZn-SOD and Se-dependent GSH-Px and kidney CuZn-SOD and GSH-Red activities were lower in DIAB rats compared to CON rats; kidney Mn-SOD and kidney Se-dependent GSH-Px activities were higher in DIAB rats than CON rats. Vanadium treatment did not cause significant alterations in the antioxidant defense system; however, tissue vandium concentrations were positively correlated to TBARS production. These results s3ow that diabetes caused significant alterations in the antioxidant defence system and that V therapy was associated with a marked deterioration in health of both control and diabetic rats.
AB - The effect of sodium metavanadate (NaVO3) consumption on trace element metabolism, components of the antioxidant defense system and lipid oxidative damage were studied in control (CON) and streptozotocin-induced diabetic (DIAB) rats. Ten days after injection, CON and DIAB rats received either 0 mM NaVO3/80 mM NaCl (0 group) or 1.2 mM NaVO3/80 mM NaCl (1.2V group) in their drinking water. DIAB groups had higher food and fluid intakes than the CON groups; vanadium (V) groups had lower food and fluid intakes than the saline groups. Vanadium therapy lowered plasma glucose concentrations of DIAB rats. The following parameters were similar among the groups: plasma Zn, Cu and Fe concentrations, plasma ceruloplasmin activity, liver Zn, Cu, Mn and Fe concentrations, kidney Mn and Fe concentrations, liver non-Se-dependent glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Red) and Mn-SOD activities, liver reduced glutathione (GSH) and oxidized glutathione (GSSG) concentrations and kidney non-Se-dependent GSH-Px activity. Kidney Zn and Cu concentrations were higher in DIAB rats than in CON rats. The CON-1.2V and DIAB-1.2V groups had V accumulation in the liver and kidney. Liver CuZn-SOD and Se-dependent GSH-Px and kidney CuZn-SOD and GSH-Red activities were lower in DIAB rats compared to CON rats; kidney Mn-SOD and kidney Se-dependent GSH-Px activities were higher in DIAB rats than CON rats. Vanadium treatment did not cause significant alterations in the antioxidant defense system; however, tissue vandium concentrations were positively correlated to TBARS production. These results s3ow that diabetes caused significant alterations in the antioxidant defence system and that V therapy was associated with a marked deterioration in health of both control and diabetic rats.
KW - Antioxidant defense
KW - Diabetes
KW - Lipid peroxidation
KW - Trace elements
KW - Vanadium
UR - http://www.scopus.com/inward/record.url?scp=0027426193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027426193&partnerID=8YFLogxK
U2 - 10.1016/0300-483X(93)90096-B
DO - 10.1016/0300-483X(93)90096-B
M3 - Article
C2 - 8248940
AN - SCOPUS:0027426193
VL - 83
SP - 115
EP - 130
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 1-3
ER -