Validation of lactate measurement in american flamingo (Phoenicopterus ruber) plasma and correlation with duration and difficulty of capture

Anne Burgdorf-Moisuk, Raymund Wack, Michael H Ziccardi, R. Scott Larsen, Katrina Hopper

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Capture myopathy and associated death have been reported with capture and restraint of greater flamingos (Phoenicopterus ruber roseus) and lesser flamingos (Phoeniconaias minor). In chickens (Gallus gallus), blood lactate concentration levels have been used as indicators of muscle damage. Lactate has also been used to predict survival in humans and dogs. The goals of this study were to validate two common methods for measuring lactate (i-STAT® and VetTest® analyzers) in flamingo plasma by comparing measurements to a reference analyzer; and to correlate blood lactate concentration levels in captured flamingos with the duration and difficulty of capture as a possible indicator of capture myopathy. Twenty-seven banked flamingo plasma samples were run in triplicate on each of the three blood analyzers. Values from the i-STAT analyzer were consistently lower than those from the ABL analyzer, while values from the VetTest were consistently higher than those from the ABL analyzer. However, there was a good level of correlation between all three analyzers. Two of the three analyzers were determined to have acceptable total allowable error levels, calculated at 3.6 for the ABL and 10.7 for the VetTest. For clinical purposes, both the i-STAT and the VetTest analyzers provide adequate evaluation of lactate levels when serial samples are measured on the same analyzer. After validating the assay, 34 captive flamingos were captured for routine examinations. Blood lactate concentration levels were positively correlated with the length of time of the individual capture, but lactate did not increase significantly as capture difficulty increased. Only one animal was considered to have a difficult capture. No flamingos demonstrated clinical signs of capture myopathy during this study. Further research is required to determine if blood lactate concentration is a useful indicator of capture myopathy.

Original languageEnglish (US)
Pages (from-to)450-458
Number of pages9
JournalJournal of Zoo and Wildlife Medicine
Volume43
Issue number3
DOIs
StatePublished - Sep 20 2012

Fingerprint

Phoenicopteridae
lactates
Lactic Acid
duration
muscular diseases
Muscular Diseases
blood
Chickens
Gallus gallus
Phoenicopterus ruber
Dogs
death
chickens
sampling
Muscles
muscles
Survival
dogs
assays
Research

Keywords

  • Blood lactate
  • Capture myopathy
  • Flamingo
  • Phoeniconaias minor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Validation of lactate measurement in american flamingo (Phoenicopterus ruber) plasma and correlation with duration and difficulty of capture. / Burgdorf-Moisuk, Anne; Wack, Raymund; Ziccardi, Michael H; Larsen, R. Scott; Hopper, Katrina.

In: Journal of Zoo and Wildlife Medicine, Vol. 43, No. 3, 20.09.2012, p. 450-458.

Research output: Contribution to journalArticle

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