Validation of an immunoassay for soluble Klotho protein: Decreased levels in diabetes and increased levels in chronic kidney disease

Sridevi Devaraj, Basir Syed, Alexander Chien, Ishwarlal Jialal

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

The Klotho gene has been identified as an aging suppressor gene that encodes a transmembrane protein, which is expressed primarily in renal tubules. There are 2 forms of Klotho, membrane and secreted. However, there is a paucity of data on levels of soluble Klotho in diseases like diabetes and kidney disease. We validated an enzyme-linked immunosorbent assay for Klotho and quantitated Klotho levels separately in patients with diabetes and also in patients with chronic kidney disease (CKD). The Klotho assay showed good precision and was linear down to 19 ng/mL. There were no significant effects on Klotho levels with the addition of common interferents such as ascorbate, triglycerides, or hemolysis; only bilirubin (250 mg/L) significantly reduced Klotho levels (P < .05). There was a significant reduction in Klotho levels in samples with glycated hemoglobin (HbA 1c) levels of 6.5% or more compared with control samples (HbA 1c < 6.5%; P < .001). We also documented significantly higher levels of Klotho with CKD. Thus, we validated an assay for Klotho and made the novel observation that levels are decreased in diabetes and increased in CKD. Copyright

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume137
Issue number3
DOIs
StatePublished - Mar 2012

Keywords

  • Aging
  • Chronic kidney disease
  • Diabetes
  • Fibroblast growth factor
  • Inflammation
  • Klotho

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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