Validation of an immunoassay for measurement of plasma total homocysteine

Karine Marangon, Dawn O'Byrne, Sridevi Devaraj, Ishwarlal Jialal

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Hyperhomocysteinemia is an evolving cardiovascular risk factor. It is imperative that a simple, precise, and accurate assay be available in the clinical laboratory. The aim of this study was to evaluate an automated fluorescence polarization immunoassay for homocysteine. The assay had excellent precision at normal and high levels (intra-assay and interassay coefficients of variation <5%). The method was linear from 0.24 to 50 μmol/L and displayed good correlation with a high-performance liquid chromatography (HPLC) method. There was no significant interference detectable in icteric and hyperlipidemic samples, but hemolysis resulted in a significant negative bias. While homocysteine levels were not increased in smokers, patients with renal failure had significantly higher levels compared with control subjects. This automated assay requires no sample preparation, displays excellent precision, shows good correlation with HPLC, and, thus, is favored over HPLC for use in the clinical laboratory. The main indications for measuring plasma homocysteine levels will be in the early diagnosis of cobalamin deficiency, patients with cardiovascular disease and few or no established risk factors, and patients with unexplained venous thromboembolic disease.

Original languageEnglish (US)
Pages (from-to)757-762
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume112
Issue number6
StatePublished - 1999
Externally publishedYes

Fingerprint

Homocysteine
Immunoassay
High Pressure Liquid Chromatography
Fluorescence Polarization Immunoassay
Hyperhomocysteinemia
Vitamin B 12
Hemolysis
Renal Insufficiency
Early Diagnosis
Cardiovascular Diseases

Keywords

  • Dialysis
  • Homocysteine
  • Immunoassay
  • Smoking

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Marangon, K., O'Byrne, D., Devaraj, S., & Jialal, I. (1999). Validation of an immunoassay for measurement of plasma total homocysteine. American Journal of Clinical Pathology, 112(6), 757-762.

Validation of an immunoassay for measurement of plasma total homocysteine. / Marangon, Karine; O'Byrne, Dawn; Devaraj, Sridevi; Jialal, Ishwarlal.

In: American Journal of Clinical Pathology, Vol. 112, No. 6, 1999, p. 757-762.

Research output: Contribution to journalArticle

Marangon, K, O'Byrne, D, Devaraj, S & Jialal, I 1999, 'Validation of an immunoassay for measurement of plasma total homocysteine', American Journal of Clinical Pathology, vol. 112, no. 6, pp. 757-762.
Marangon, Karine ; O'Byrne, Dawn ; Devaraj, Sridevi ; Jialal, Ishwarlal. / Validation of an immunoassay for measurement of plasma total homocysteine. In: American Journal of Clinical Pathology. 1999 ; Vol. 112, No. 6. pp. 757-762.
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