Validation of an automated ELISA system for detection of antibodies to Aleutian mink disease virus using blood samples collected in filter paper strips

Anna Knuuttila, Pirjo Aronen, Majvor Eerola, Ian Gardner, Anna Maija K Virtala, Olli Vapalahti

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Aleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to validate an AMDV-VP2 -recombinant antigen ELISA, which we developed earlier, in an automated assay format for the detection of anti-AMDV antibodies in mink blood and to determine the accuracy of this test compared with the reference standard (counter-current immunoelectrophoresis, CIEP).

Methods. A blood sampling method based on filter paper 12-strips (blood combs) and a device to introduce these strips to an ELISA plate for elution of the samples were developed. Blood and serum samples were collected from 761 mink from two farms with low (2%) and high (81%) seroprevalences of AMDV infection in 2008. ELISA sensitivity and specificity were estimated with a Bayesian 2-test 2-population model that allowed for conditional dependence between CIEP and ELISA. Agreement between the two tests was assessed with kappa statistic and proportion agreement.

Results: The sensitivity and specificity of the automated ELISA system were estimated to be 96.2% and 98.4%, respectively. Agreement between CIEP and ELISA was high, with a kappa value of 0.976 and overall proportion agreement of 98.8%.

Conclusions: The automated ELISA system combined with blood comb sampling is an accurate test format for the detection of anti-AMDV antibodies in mink blood and offers several advantages, including improved blood sampling and data handling, fast sample throughput time, and reductions in costs and labour inputs.

Original languageEnglish (US)
Article number141
JournalVirology Journal
Volume11
Issue number1
DOIs
StatePublished - Aug 8 2014
Externally publishedYes

Fingerprint

Aleutian Mink Disease Virus
Enzyme-Linked Immunosorbent Assay
Mink
Antibodies
Immunoelectrophoresis
Comb and Wattles
Immune Complex Diseases
Disease Eradication
Sensitivity and Specificity
Seroepidemiologic Studies
Virus Diseases
Finland
Antigens
Costs and Cost Analysis
Equipment and Supplies

Keywords

  • Aleutian mink disease virus
  • Bayesian analysis
  • Carnivore amdoparvovirus 1
  • Counter-current immunoelectrophoresis
  • Enzyme-linked immunosorbent assay
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Validation of an automated ELISA system for detection of antibodies to Aleutian mink disease virus using blood samples collected in filter paper strips. / Knuuttila, Anna; Aronen, Pirjo; Eerola, Majvor; Gardner, Ian; Virtala, Anna Maija K; Vapalahti, Olli.

In: Virology Journal, Vol. 11, No. 1, 141, 08.08.2014.

Research output: Contribution to journalArticle

Knuuttila, Anna ; Aronen, Pirjo ; Eerola, Majvor ; Gardner, Ian ; Virtala, Anna Maija K ; Vapalahti, Olli. / Validation of an automated ELISA system for detection of antibodies to Aleutian mink disease virus using blood samples collected in filter paper strips. In: Virology Journal. 2014 ; Vol. 11, No. 1.
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abstract = "Background: Aleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to validate an AMDV-VP2 -recombinant antigen ELISA, which we developed earlier, in an automated assay format for the detection of anti-AMDV antibodies in mink blood and to determine the accuracy of this test compared with the reference standard (counter-current immunoelectrophoresis, CIEP).Methods. A blood sampling method based on filter paper 12-strips (blood combs) and a device to introduce these strips to an ELISA plate for elution of the samples were developed. Blood and serum samples were collected from 761 mink from two farms with low (2{\%}) and high (81{\%}) seroprevalences of AMDV infection in 2008. ELISA sensitivity and specificity were estimated with a Bayesian 2-test 2-population model that allowed for conditional dependence between CIEP and ELISA. Agreement between the two tests was assessed with kappa statistic and proportion agreement.Results: The sensitivity and specificity of the automated ELISA system were estimated to be 96.2{\%} and 98.4{\%}, respectively. Agreement between CIEP and ELISA was high, with a kappa value of 0.976 and overall proportion agreement of 98.8{\%}.Conclusions: The automated ELISA system combined with blood comb sampling is an accurate test format for the detection of anti-AMDV antibodies in mink blood and offers several advantages, including improved blood sampling and data handling, fast sample throughput time, and reductions in costs and labour inputs.",
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