Validation of a time-resolved fluorescence spectroscopy apparatus using a rabbit atherosclerosis model

Qiyin Fang, Javier Jo, Thanassis Papaioannou, Amir Dorafshar, Todd Reil, Jian Hua Qiao, Michael C. Fishbein, J. A. Freischlag, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Time-resolved laser-induced fluorescence spectroscopy (tr-LIFS) has been studied as a potential tool for in vivo diagnosis of atherosclerotic lesions. This study is to evaluate the potential of a compact fiber-optics based tr-LIFS instrument developed in our laboratory for in vivo analysis of atherosclerotic plaque composition. Time-resolved fluorescence spectroscopy studies were performed in vivo on fifteen New Zealand White rabbits (atherosclerotic: N=8, control: N=7). Time-resolved fluorescence spectra were acquired (range: 360-600 nm, increment: 5 nm, total acquisition time: 65 s) from normal aorta wall and lesions in the abdominal aorta. Data were analyzed in terms of fluorescence emission spectra and wavelength specific lifetimes. Following trichrome staining, tissue specimens were analyzed histopathologically in terms of intima/media thickness and biochemical composition (collagen, elastin, foam cells, and etc). Based on intimal thickness, the lesions were divided into thin and thick lesions. Each group was further separated into two categories: collagen rich lesions and foam cell rich lesions based on their biochemical composition. The obtained spectral and time domain fluorescence signatures were subsequently correlated to the histopathological findings. The results have shown that time-domain fluorescence spectral features can be used in vivo to separate atherosclerotic lesions from normal aorta wall as well discrimination within certain types of lesions.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsK.E. Bartels, L.S. Bass, W.T.W. Riese, K.W. Gregory, H. Hirschberg, A. Katzir, N. Kollias, S.J. Madsen, R.S. Malek
Number of pages6
StatePublished - 2004
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV - San Jose, CA, United States
Duration: Jan 24 2004Jan 27 2004


OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Country/TerritoryUnited States
CitySan Jose, CA


  • Animal model
  • Atherosclerosis
  • Clinical diagnostics
  • Collagen
  • Elastin
  • Fiber-optics
  • Fluorescence
  • Foam cells
  • In-vivo
  • Laser-induced
  • New Zealand White rabbit
  • Noninvasive tissue characterization
  • Smooth muscle cells
  • Time-resolved

ASJC Scopus subject areas

  • Engineering(all)


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