Abstract
Objective: To evaluate high frequency (40 MHz) B-mode ultrasound for the detection of osteoarthritis (OA) lesions of varying severity in an animal model of OA. Design: Ultrasound biomicroscopy (UBM) was performed on the femoral articular surface of adult rabbits with unilateral transection of the anterior cruciate ligament at 4, 8 and 12 weeks post-surgery and on control rabbits. The articular cartilage was examined and graded macroscopically and histologically for OA lesions. Histological examination was used as a reference to determine sensitivity and specificity of ultrasonographic and macroscopic examination regarding fibrillation and ulceration of articular cartilage. Results: Identification of slight surface irregularities was made possible with UBM. The sensitivity and specificity of UBM were 92.3% and 96.4%, respectively, to detect histological fibrillation and 90.9% and 97.6%, respectively, to identify histological ulceration. Macroscopic examination using India Ink had a sensitivity and specificity of 80% and 96.4%, respectively, for fibrillation and 90.9% and 90.5%, respectively, for ulceration when compared to histology. A high correlation (rsp = 0.90) was found between ultrasonographic and histological scores. Conclusions: UBM of articular cartilage reflects histological structure and can accurately detect early changes such as fibrillation. UBM has the potential to be a valuable tool for the in vivo identification of early lesions of OA and for monitoring the disease or efficacy of novel therapy if it can be packaged in a minimally invasive format suitable for intra-articular imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 171-179 |
| Number of pages | 9 |
| Journal | Osteoarthritis and Cartilage |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2005 |
| Externally published | Yes |
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Keywords
- Cartilage
- Histology
- Osteoarthritis
- Ultrasound biomicroscopy
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
Cite this
Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model. / Spriet, Mathieu; Girard, Christiane A.; Foster, Stuart F.; Harasiewicz, Kasia; Holdsworth, David W.; Laverty, Sheila.
In: Osteoarthritis and Cartilage, Vol. 13, No. 2, 02.2005, p. 171-179.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model
AU - Spriet, Mathieu
AU - Girard, Christiane A.
AU - Foster, Stuart F.
AU - Harasiewicz, Kasia
AU - Holdsworth, David W.
AU - Laverty, Sheila
PY - 2005/2
Y1 - 2005/2
N2 - Objective: To evaluate high frequency (40 MHz) B-mode ultrasound for the detection of osteoarthritis (OA) lesions of varying severity in an animal model of OA. Design: Ultrasound biomicroscopy (UBM) was performed on the femoral articular surface of adult rabbits with unilateral transection of the anterior cruciate ligament at 4, 8 and 12 weeks post-surgery and on control rabbits. The articular cartilage was examined and graded macroscopically and histologically for OA lesions. Histological examination was used as a reference to determine sensitivity and specificity of ultrasonographic and macroscopic examination regarding fibrillation and ulceration of articular cartilage. Results: Identification of slight surface irregularities was made possible with UBM. The sensitivity and specificity of UBM were 92.3% and 96.4%, respectively, to detect histological fibrillation and 90.9% and 97.6%, respectively, to identify histological ulceration. Macroscopic examination using India Ink had a sensitivity and specificity of 80% and 96.4%, respectively, for fibrillation and 90.9% and 90.5%, respectively, for ulceration when compared to histology. A high correlation (rsp = 0.90) was found between ultrasonographic and histological scores. Conclusions: UBM of articular cartilage reflects histological structure and can accurately detect early changes such as fibrillation. UBM has the potential to be a valuable tool for the in vivo identification of early lesions of OA and for monitoring the disease or efficacy of novel therapy if it can be packaged in a minimally invasive format suitable for intra-articular imaging.
AB - Objective: To evaluate high frequency (40 MHz) B-mode ultrasound for the detection of osteoarthritis (OA) lesions of varying severity in an animal model of OA. Design: Ultrasound biomicroscopy (UBM) was performed on the femoral articular surface of adult rabbits with unilateral transection of the anterior cruciate ligament at 4, 8 and 12 weeks post-surgery and on control rabbits. The articular cartilage was examined and graded macroscopically and histologically for OA lesions. Histological examination was used as a reference to determine sensitivity and specificity of ultrasonographic and macroscopic examination regarding fibrillation and ulceration of articular cartilage. Results: Identification of slight surface irregularities was made possible with UBM. The sensitivity and specificity of UBM were 92.3% and 96.4%, respectively, to detect histological fibrillation and 90.9% and 97.6%, respectively, to identify histological ulceration. Macroscopic examination using India Ink had a sensitivity and specificity of 80% and 96.4%, respectively, for fibrillation and 90.9% and 90.5%, respectively, for ulceration when compared to histology. A high correlation (rsp = 0.90) was found between ultrasonographic and histological scores. Conclusions: UBM of articular cartilage reflects histological structure and can accurately detect early changes such as fibrillation. UBM has the potential to be a valuable tool for the in vivo identification of early lesions of OA and for monitoring the disease or efficacy of novel therapy if it can be packaged in a minimally invasive format suitable for intra-articular imaging.
KW - Cartilage
KW - Histology
KW - Osteoarthritis
KW - Ultrasound biomicroscopy
UR - http://www.scopus.com/inward/record.url?scp=13544262607&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13544262607&partnerID=8YFLogxK
U2 - 10.1016/j.joca.2004.10.022
DO - 10.1016/j.joca.2004.10.022
M3 - Article
C2 - 15694579
AN - SCOPUS:13544262607
VL - 13
SP - 171
EP - 179
JO - Osteoarthritis and Cartilage
JF - Osteoarthritis and Cartilage
SN - 1063-4584
IS - 2
ER -