Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model

Mathieu Spriet, Christiane A. Girard, Stuart F. Foster, Kasia Harasiewicz, David W. Holdsworth, Sheila Laverty

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objective: To evaluate high frequency (40 MHz) B-mode ultrasound for the detection of osteoarthritis (OA) lesions of varying severity in an animal model of OA. Design: Ultrasound biomicroscopy (UBM) was performed on the femoral articular surface of adult rabbits with unilateral transection of the anterior cruciate ligament at 4, 8 and 12 weeks post-surgery and on control rabbits. The articular cartilage was examined and graded macroscopically and histologically for OA lesions. Histological examination was used as a reference to determine sensitivity and specificity of ultrasonographic and macroscopic examination regarding fibrillation and ulceration of articular cartilage. Results: Identification of slight surface irregularities was made possible with UBM. The sensitivity and specificity of UBM were 92.3% and 96.4%, respectively, to detect histological fibrillation and 90.9% and 97.6%, respectively, to identify histological ulceration. Macroscopic examination using India Ink had a sensitivity and specificity of 80% and 96.4%, respectively, for fibrillation and 90.9% and 90.5%, respectively, for ulceration when compared to histology. A high correlation (rsp = 0.90) was found between ultrasonographic and histological scores. Conclusions: UBM of articular cartilage reflects histological structure and can accurately detect early changes such as fibrillation. UBM has the potential to be a valuable tool for the in vivo identification of early lesions of OA and for monitoring the disease or efficacy of novel therapy if it can be packaged in a minimally invasive format suitable for intra-articular imaging.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalOsteoarthritis and Cartilage
Volume13
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Acoustic Microscopy
Osteoarthritis
Animals
Animal Models
Ultrasonics
Cartilage
Articular Cartilage
Sensitivity and Specificity
Joints
Rabbits
Histology
Anterior Cruciate Ligament
Ligaments
Thigh
Ink
Surgery
Imaging techniques
Monitoring

Keywords

  • Cartilage
  • Histology
  • Osteoarthritis
  • Ultrasound biomicroscopy

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model. / Spriet, Mathieu; Girard, Christiane A.; Foster, Stuart F.; Harasiewicz, Kasia; Holdsworth, David W.; Laverty, Sheila.

In: Osteoarthritis and Cartilage, Vol. 13, No. 2, 02.2005, p. 171-179.

Research output: Contribution to journalArticle

Spriet, Mathieu ; Girard, Christiane A. ; Foster, Stuart F. ; Harasiewicz, Kasia ; Holdsworth, David W. ; Laverty, Sheila. / Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model. In: Osteoarthritis and Cartilage. 2005 ; Vol. 13, No. 2. pp. 171-179.
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