Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle

M. Mackett, Tilahun Yilma, J. K. Rose, B. Moss

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148 Scopus citations

Abstract

Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.

Original languageEnglish (US)
Pages (from-to)433-435
Number of pages3
JournalScience
Volume227
Issue number4685
StatePublished - 1985
Externally publishedYes

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    Mackett, M., Yilma, T., Rose, J. K., & Moss, B. (1985). Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle. Science, 227(4685), 433-435.