Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle

M. Mackett, Tilahun Yilma, J. K. Rose, B. Moss

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.

Original languageEnglish (US)
Pages (from-to)433-435
Number of pages3
JournalScience
Volume227
Issue number4685
StatePublished - 1985
Externally publishedYes

Fingerprint

Vesicular Stomatitis
Vaccinia virus
Immunization
Viruses
Genes
Neutralizing Antibodies
Vaccination
Horse Diseases
Cattle Diseases
Vaccinia
Encephalitis
GTP-Binding Proteins
Swine
Genome
Messenger RNA
Peptides
DNA

ASJC Scopus subject areas

  • General

Cite this

Vaccinia virus recombinants : Expression of VSV genes and protective immunization of mice and cattle. / Mackett, M.; Yilma, Tilahun; Rose, J. K.; Moss, B.

In: Science, Vol. 227, No. 4685, 1985, p. 433-435.

Research output: Contribution to journalArticle

Mackett, M. ; Yilma, Tilahun ; Rose, J. K. ; Moss, B. / Vaccinia virus recombinants : Expression of VSV genes and protective immunization of mice and cattle. In: Science. 1985 ; Vol. 227, No. 4685. pp. 433-435.
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