Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle

M. Mackett; Tilahun Yilma; J. K. Rose; B. Moss

Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle

M. Mackett, Tilahun Yilma, J. K. Rose, B. Moss

Research output: Contribution to journal › Article › peer-review

Abstract

Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.

Original language	English (US)
Pages (from-to)	433-435
Number of pages	3
Journal	Science
Volume	227
Issue number	4685
State	Published - 1985
Externally published	Yes

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title = "Vaccinia virus recombinants: Expression of VSV genes and protective immunization of mice and cattle",

abstract = "Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.",

author = "M. Mackett and Tilahun Yilma and Rose, {J. K.} and B. Moss",

year = "1985",

language = "English (US)",

volume = "227",

pages = "433--435",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4685",

}

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T1 - Vaccinia virus recombinants

T2 - Expression of VSV genes and protective immunization of mice and cattle

AU - Mackett, M.

AU - Yilma, Tilahun

AU - Rose, J. K.

AU - Moss, B.

PY - 1985

Y1 - 1985

N2 - Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.

AB - Vesicular stomatitis virus (VSV) causes a contagious disease of horses, cattle, and pigs. When DNA copies of messenger RNA's for the G or N proteins of VSV were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized VSV polypeptides. After intradermal vaccination with live recombinant virus expressing the G protein, mice produced VSV-neutralizing antibodies and were protected against lethal encephalitis upon intravenous challenge with VSV. In cattle, the degree of protection against intradermalingually injected VSV was correlated with the level of neutralizing antibody produced following vaccination.

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