Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-α plasmids preserves global CD4 T lymphocyte function after challenge with FIV

Saipiroon Maksaereekul, Robert A. Dubie, Xiaoying Shen, Hung Kieu, Gregg A. Dean, Ellen E. Sparger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Feline immunodeficiency virus (FIV) DNA vaccine approaches that included a vif-deleted FIV provirus (FIV-pPPRΔvif) and feline cytokine expression plasmids were tested for immunogenicity and efficacy by immunization of specific pathogen free cats. Vaccine protocols included FIV-pPPRΔvif plasmid alone; a combination of FIV-pPPRΔvif DNA and feline granulocyte macrophage-colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-α expression plasmids; or a combination of FIV-pPPRΔvif and feline interleukin (IL)-15 plasmids. Cats immunized with FIV-pPPRΔvif, GM-CSF and TNF-α plasmids demonstrated an increased frequency of FIV-specific T cell proliferation responses compared to other vaccine groups. Immunization with FIV-pPPRΔvif and IL-15 plasmids was distinguished from other vaccine protocols by the induction of antiviral antibodies. Suppression of virus loads was not observed for any of the FIV-pPPRΔvif DNA vaccine protocols after challenge with the FIV-PPR isolate. However, prior immunization with FIV-pPPRΔvif, GM-CSF, and TNF-α plasmids resulted in preservation of CD4 T cell functions, including mitogen-induced cytokine expression and antigen-specific proliferation upon infection with FIV. These findings justify further examination of cytokine combinations as adjuvants for lentiviral DNA vaccines.

Original languageEnglish (US)
Pages (from-to)3754-3765
Number of pages12
JournalVaccine
Volume27
Issue number28
DOIs
StatePublished - Jun 8 2009

Fingerprint

Feline Immunodeficiency Virus
proviruses
granulocyte-macrophage colony-stimulating factor
Proviruses
Feline immunodeficiency virus
tumor necrosis factors
Granulocyte-Macrophage Colony-Stimulating Factor
preserves
plasmids
Vaccination
Plasmids
Tumor Necrosis Factor-alpha
T-lymphocytes
vaccination
T-Lymphocytes
DNA Vaccines
cats
Felidae
recombinant vaccines
Interleukin-15

Keywords

  • Cytokine adjuvants
  • DNA vaccine
  • FIV

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-α plasmids preserves global CD4 T lymphocyte function after challenge with FIV. / Maksaereekul, Saipiroon; Dubie, Robert A.; Shen, Xiaoying; Kieu, Hung; Dean, Gregg A.; Sparger, Ellen E.

In: Vaccine, Vol. 27, No. 28, 08.06.2009, p. 3754-3765.

Research output: Contribution to journalArticle

Maksaereekul, Saipiroon ; Dubie, Robert A. ; Shen, Xiaoying ; Kieu, Hung ; Dean, Gregg A. ; Sparger, Ellen E. / Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-α plasmids preserves global CD4 T lymphocyte function after challenge with FIV. In: Vaccine. 2009 ; Vol. 27, No. 28. pp. 3754-3765.
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