v-Raf activates transcription of growth-responsive promoters via GC-rich sequences that bind the transcription factor Sp1

R. J. Miltenberger, P. J. Farnham, D. E. Smith, J. M. Stommel, M. M. Cornwell

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The serine/threonine kinase, Raf-1, is a component of intracellular signaling pathways that control responses to extracellular stimuli. Previously, we have shown that serum-induced transcription from the murine rep-3b and human mdr1 promoters is Raf-dependent and that the activated Raf kinase, v-Raf, induces transcription of mdr1 via a GC-rich element. We now demonstrate that GC-rich sequences in the rep-3b promoter are both necessary and sufficient for induction by v-Raf. The GC-rich, v-Raf-responsive elements of rep-3b and mdr1 bind the general transcription factor Sp1 in electromobility shift assays. Mutation of a minimal GC-rich element abolished inducibility of v-Raf and eliminated binding by the transcription factor Sp1. However, Sp1 binding activity following serum stimulation of quiescent NIH 3T3 cells was unchanged, suggesting that mitogenic signals may stimulate the transactivation potential of prebound Sp1.

Original languageEnglish (US)
Pages (from-to)549-556
Number of pages8
JournalCell Growth and Differentiation
Volume6
Issue number5
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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