Utility of residual AutoCyte cervical cytology samples for image analysis

John W Bishop, Dale A. Cheuvront, Robert J. Elston

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

OBJECTIVE: To ascertain the utility of residual liquid-preserved cervical cytology samples for DNA profiling studies. STUDY DESIGN: Ninety- nine liquid-preserved cervical cytology samples from a high-risk population were received two months after the initial diagnosis at another laboratory. Each residual sample was processed to yield one Papanicolaou-stained and one Feulgen-thionin-stained slide. The former were examined manually without knowledge of the submitted findings and were also examined on the AutoCyte SCREEN interactive system. DNA profiling of >185 cells per case was performed on an RPW image analyzer. Results were compared with submitted diagnoses. RESULTS: The 99 cases included 59 normals, 9 ASCUS/AGCUS, 16 HSIL, 12 carcinomas and 3 unsatisfactory. Interlaboratory agreement between residual and initial samples was good (unweighted kappa = .609). Abnormals were characterized by 2C deviation indices >2.0 and 5C exceeding rates >4.37%. AutoCyte SCREEN system examination of residual slides of high grade abnormalities was 100% sensitive and 40% specific. CONCLUSION: Residual AutoCyte cervical cytology samples are stable and yield reproducible results for routine and ancillary studies of cervical cytologic abnormalities. The AutoCyte SCREEN system was 100% sensitive for high grade abnormalities in this enriched sample, even when operating on residual material.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalActa Cytologica
Volume43
Issue number1
StatePublished - 1999
Externally publishedYes

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Keywords

  • AutoCyte
  • Cervix neoplasms
  • Computer-assisted
  • Image analysis
  • Liquid-based thin layers

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

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