Abstract
OBJECTIVE: To ascertain the utility of residual liquid-preserved cervical cytology samples for DNA profiling studies. STUDY DESIGN: Ninety- nine liquid-preserved cervical cytology samples from a high-risk population were received two months after the initial diagnosis at another laboratory. Each residual sample was processed to yield one Papanicolaou-stained and one Feulgen-thionin-stained slide. The former were examined manually without knowledge of the submitted findings and were also examined on the AutoCyte SCREEN interactive system. DNA profiling of >185 cells per case was performed on an RPW image analyzer. Results were compared with submitted diagnoses. RESULTS: The 99 cases included 59 normals, 9 ASCUS/AGCUS, 16 HSIL, 12 carcinomas and 3 unsatisfactory. Interlaboratory agreement between residual and initial samples was good (unweighted kappa = .609). Abnormals were characterized by 2C deviation indices >2.0 and 5C exceeding rates >4.37%. AutoCyte SCREEN system examination of residual slides of high grade abnormalities was 100% sensitive and 40% specific. CONCLUSION: Residual AutoCyte cervical cytology samples are stable and yield reproducible results for routine and ancillary studies of cervical cytologic abnormalities. The AutoCyte SCREEN system was 100% sensitive for high grade abnormalities in this enriched sample, even when operating on residual material.
Original language | English (US) |
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Pages (from-to) | 39-46 |
Number of pages | 8 |
Journal | Acta Cytologica |
Volume | 43 |
Issue number | 1 |
State | Published - 1999 |
Externally published | Yes |
Keywords
- AutoCyte
- Cervix neoplasms
- Computer-assisted
- Image analysis
- Liquid-based thin layers
ASJC Scopus subject areas
- Anatomy
- Cell Biology
- Histology