Abstract
The diagnostic utility of flow cytometry in the evaluation of cytopenias and in the differential diagnosis of low -grade versus high-grade myelodysplastic syndrome (MDS) is not widely appreciated. In this report, we measured granulocyte CD10/control fluorescence ratio in 29 patients with MDS & chronic myelomonocytic leukemia (CMML) using peripheral blood (PB) flow cytometry (FC). We found a lower ratio in high-grade MDS and CMML (mean ratio of 2.2 ± 0.7) vs. low-grade MDS (3.65 ± 0.9) and 16 cytopenic controls without MDS (3.67 ± 0.65; p<0.001). The sensitivity and specificity of CD10 ratio <3 for the group that included the high risk MDS and CMML patients were 87.5% and 100%, respectively. Our data suggests that FC of PB may be helpful in the work-up of patients with cytopenias and in the differential diagnosis of low-grade vs. high-grade MDS.
Original language | English (US) |
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Pages (from-to) | 224-230 |
Number of pages | 7 |
Journal | International Journal of Clinical and Experimental Pathology |
Volume | 5 |
Issue number | 3 |
State | Published - May 23 2012 |
Keywords
- Bone marrow
- CD10
- Flow cytometry
- High-grade MDS
- Low-grade MDS
- Myelodysplastic syndromes
- Peripheral blood
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology