Abstract
A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 140-142 |
| Number of pages | 3 |
| Journal | Journal of Wildlife Diseases |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2014 |
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
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Cite this
Roug, A., Geoghegan, C., Wellington, E., Smith, W. A., Travis, E., Porter, D., Cooper, D., Clifford, D. L., Mazet, J. A., & Parsons, S. (2014). Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer). Journal of Wildlife Diseases, 50(1), 140-142. https://doi.org/10.7589/2013-07-159