Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer)

Annette Roug, Claire Geoghegan, Elizabeth Wellington, Woutrina A Smith, Emma Travis, David Porter, David Cooper, Deana L. Clifford, Jonna A Mazet, Sven Parsons

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.

Original languageEnglish (US)
Pages (from-to)140-142
Number of pages3
JournalJournal of Wildlife Diseases
Volume50
Issue number1
DOIs
StatePublished - Jan 2014

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

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