Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer)

Annette Roug, Claire Geoghegan, Elizabeth Wellington, Woutrina A Smith, Emma Travis, David Porter, David Cooper, Deana L. Clifford, Jonna A Mazet, Sven Parsons

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.

Original languageEnglish (US)
Pages (from-to)140-142
Number of pages3
JournalJournal of Wildlife Diseases
Volume50
Issue number1
DOIs
StatePublished - Jan 2014

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Syncerus caffer
Mycobacterium bovis
quantitative polymerase chain reaction
bovine tuberculosis
feces
infection
genome
sampling
cells
protocol
detection

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer). / Roug, Annette; Geoghegan, Claire; Wellington, Elizabeth; Smith, Woutrina A; Travis, Emma; Porter, David; Cooper, David; Clifford, Deana L.; Mazet, Jonna A; Parsons, Sven.

In: Journal of Wildlife Diseases, Vol. 50, No. 1, 01.2014, p. 140-142.

Research output: Contribution to journalArticle

Roug, Annette ; Geoghegan, Claire ; Wellington, Elizabeth ; Smith, Woutrina A ; Travis, Emma ; Porter, David ; Cooper, David ; Clifford, Deana L. ; Mazet, Jonna A ; Parsons, Sven. / Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer). In: Journal of Wildlife Diseases. 2014 ; Vol. 50, No. 1. pp. 140-142.
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