Abstract
A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 140-142 |
| Number of pages | 3 |
| Journal | Journal of Wildlife Diseases |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2014 |
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ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
Cite this
Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer). / Roug, Annette; Geoghegan, Claire; Wellington, Elizabeth; Smith, Woutrina A; Travis, Emma; Porter, David; Cooper, David; Clifford, Deana L.; Mazet, Jonna A; Parsons, Sven.
In: Journal of Wildlife Diseases, Vol. 50, No. 1, 01.2014, p. 140-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo (Syncerus caffer)
AU - Roug, Annette
AU - Geoghegan, Claire
AU - Wellington, Elizabeth
AU - Smith, Woutrina A
AU - Travis, Emma
AU - Porter, David
AU - Cooper, David
AU - Clifford, Deana L.
AU - Mazet, Jonna A
AU - Parsons, Sven
PY - 2014/1
Y1 - 2014/1
N2 - A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
AB - A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis-infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette-Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
UR - http://www.scopus.com/inward/record.url?scp=84892426766&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84892426766&partnerID=8YFLogxK
U2 - 10.7589/2013-07-159
DO - 10.7589/2013-07-159
M3 - Article
C2 - 24171565
AN - SCOPUS:84892426766
VL - 50
SP - 140
EP - 142
JO - Journal of Wildlife Diseases
JF - Journal of Wildlife Diseases
SN - 0090-3558
IS - 1
ER -