Uterine mast cells and immunoglobulin-E antibody responses during clearance of tritrichomonas foetus

L. B. Corbeil, C. M. Campero, J. C. Rhyan, Mark L Anderson, Laurel J Gershwin, D. W. Agnew, L. Munson, Robert Bondurant

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We showed earlier that Tritrichomonas foetus-specific bovine immunoglobulin (Ig)G1 and IgA antibodies in uterine and vaginal secretions are correlated with clearance of this sexually transmitted infection. Eosinophils have been noted in previous studies of bovine trichomoniasis but the role of mast cells and IgE responses have not been reported. The hypothesis that IgE and mast cell degranulation play a role in clearance was tested in 25 virgin heifers inseminated experimentally and infected intravaginally with T. foetus strain D1 at estrus and cultured weekly. Groups were euthanatized at 3, 6, 9, or 12 weeks, when tissues were fixed and secretions were collected for culture and antibody analysis. Immunohistochemistry using a monoclonal antibody to a soluble lipophosphoglycan (LPG)-containing surface antigen (TF1.17) demonstrated antigen uptake by uterine epithelial cells. Lymphoid nodules were detected below antigen-positive epithelium. Little IgG2 antibody was detected but IgG1, IgA, IgM, and IgE T. foetus-specific antibodies increased in uterine secretions at weeks 6 and 9 after infection. This was inversely proportional to subepithelial mast cells numbers and most animals cleared the infection by the sampling time after the lowest mast cell count. Furthermore, soluble antigen was found in uterine epithelium above inductive sites (lymphoid nodules). Cross-linking of IgE on mast cells by antigen and perhaps LPG triggering appears to have resulted in degranulation. Released cytokines may account for production of predominantly Th2 (IgG1 and IgE) and IgA antibody responses, which are related to clearance of the infection.

Original languageEnglish (US)
Pages (from-to)282-290
Number of pages9
JournalVeterinary Pathology
Volume42
Issue number3
DOIs
StatePublished - May 2005

Fingerprint

Tritrichomonas foetus
immunoglobulin E
mast cells
Mast Cells
Immunoglobulin E
Antibody Formation
antibodies
Immunoglobulin A
antigens
Antigens
Antibodies
Immunoglobulin G
secretion
epithelium
Epithelium
Cell Count
Infection
infection
trichomoniasis
sexually transmitted diseases

Keywords

  • Antibodies
  • Bovine disease
  • IgE
  • Mast cells
  • Tritrichomonas foetus
  • Uterus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Uterine mast cells and immunoglobulin-E antibody responses during clearance of tritrichomonas foetus. / Corbeil, L. B.; Campero, C. M.; Rhyan, J. C.; Anderson, Mark L; Gershwin, Laurel J; Agnew, D. W.; Munson, L.; Bondurant, Robert.

In: Veterinary Pathology, Vol. 42, No. 3, 05.2005, p. 282-290.

Research output: Contribution to journalArticle

Corbeil, L. B. ; Campero, C. M. ; Rhyan, J. C. ; Anderson, Mark L ; Gershwin, Laurel J ; Agnew, D. W. ; Munson, L. ; Bondurant, Robert. / Uterine mast cells and immunoglobulin-E antibody responses during clearance of tritrichomonas foetus. In: Veterinary Pathology. 2005 ; Vol. 42, No. 3. pp. 282-290.
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