Using retroviruses as a mutagenesis tool to explore the zebrafish genome

Li-En Jao, Lisette Maddison, Wenbiao Chen, Shawn M. Burgess

Research output: Contribution to journalReview article

29 Scopus citations

Abstract

We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.

Original languageEnglish (US)
Pages (from-to)427-443
Number of pages17
JournalBriefings in Functional Genomics and Proteomics
Volume7
Issue number6
DOIs
StatePublished - 2008
Externally publishedYes

Keywords

  • Enhancer traps
  • Gene traps
  • Genetics
  • Linker-mediated PCR
  • Moloney murine leukemia virus
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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