Abstract
We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 427-443 |
| Number of pages | 17 |
| Journal | Briefings in Functional Genomics and Proteomics |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2008 |
| Externally published | Yes |
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Keywords
- Enhancer traps
- Gene traps
- Genetics
- Linker-mediated PCR
- Moloney murine leukemia virus
- Zebrafish
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
Cite this
Using retroviruses as a mutagenesis tool to explore the zebrafish genome. / Jao, Li-En; Maddison, Lisette; Chen, Wenbiao; Burgess, Shawn M.
In: Briefings in Functional Genomics and Proteomics, Vol. 7, No. 6, 2008, p. 427-443.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Using retroviruses as a mutagenesis tool to explore the zebrafish genome
AU - Jao, Li-En
AU - Maddison, Lisette
AU - Chen, Wenbiao
AU - Burgess, Shawn M.
PY - 2008
Y1 - 2008
N2 - We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.
AB - We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.
KW - Enhancer traps
KW - Gene traps
KW - Genetics
KW - Linker-mediated PCR
KW - Moloney murine leukemia virus
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=58149387573&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149387573&partnerID=8YFLogxK
U2 - 10.1093/bfgp/eln038
DO - 10.1093/bfgp/eln038
M3 - Review article
C2 - 18977782
AN - SCOPUS:58149387573
VL - 7
SP - 427
EP - 443
JO - Briefings in Functional Genomics
JF - Briefings in Functional Genomics
SN - 2041-2649
IS - 6
ER -