Using noninvasive multispectral imaging to quantitatively assess tissue vasculature

Abby Vogel, Victor V. Chernomordik, Jason D. Riley, Moinuddin Hassan, Franck Amyot, Bahar Dasgeb, Stavros G. Demos, Randall Pursley, Richard F. Little, Robert Yarchoan, Yang Tao, Amir H. Gandjbakhche

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


This research describes a noninvasive, noncontact method used to quantitatively analyze the functional characteristics of tissue. Multispectral images collected at several near-infrared wavelengths are input into a mathematical optical skin model that considers the contributions from different analytes in the epidermis and dermis skin layers. Through a reconstruction algorithm, we can quantify the percent of blood in a given area of tissue and the fraction of that blood that is oxygenated. Imaging normal tissue confirms previously reported values for the percent of blood in tissue and the percent of blood that is oxygenated in tissue and surrounding vasculature, for the normal state and when ischemia is induced. This methodology has been applied to assess vascular Kaposi's sarcoma lesions and the surrounding tissue before and during experimental therapies. The multispectral imaging technique has been combined with laser Doppler imaging to gain additional information. Results indicate that these techniques are able to provide quantitative and functional information about tissue changes during experimental drug therapy and investigate progression of disease before changes are visibly apparent, suggesting a potential for them to be used as complementary imaging techniques to clinical assessment.

Original languageEnglish (US)
Article number051604
JournalJournal of Biomedical Optics
Issue number5
StatePublished - 2007
Externally publishedYes


  • Blood volume
  • Drug therapy monitoring
  • Functional information
  • Multispectral imaging
  • Quantitative assessment
  • Tissue oxygenation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials


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