Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California

Michele T Jay-Russell, Robert E. Mandrell, Jean Yuan, Anna Bates, Rosa Manalac, Janet Mohle-Boetani, Akiko Kimura, Janice Lidgard, William G. Miller

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7%) bovine fecal, 12 of 20 (60%) flush alley water, and 1 of 20 (5%) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6%) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalJournal of Clinical Microbiology
Volume51
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Campylobacter jejuni
Disease Outbreaks
Membrane Proteins
Milk
Campylobacter Infections
Multilocus Sequence Typing
Molecular Typing
Colostrum
Water
Triage
Prisons
Pulsed Field Gel Electrophoresis
Genes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California. / Jay-Russell, Michele T; Mandrell, Robert E.; Yuan, Jean; Bates, Anna; Manalac, Rosa; Mohle-Boetani, Janet; Kimura, Akiko; Lidgard, Janice; Miller, William G.

In: Journal of Clinical Microbiology, Vol. 51, No. 1, 01.2013, p. 195-201.

Research output: Contribution to journalArticle

Jay-Russell, Michele T ; Mandrell, Robert E. ; Yuan, Jean ; Bates, Anna ; Manalac, Rosa ; Mohle-Boetani, Janet ; Kimura, Akiko ; Lidgard, Janice ; Miller, William G. / Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, No. 1. pp. 195-201.
@article{7270f246737a480a9da7e3827081961a,
title = "Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California",
abstract = "We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7{\%}) bovine fecal, 12 of 20 (60{\%}) flush alley water, and 1 of 20 (5{\%}) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6{\%}) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.",
author = "Jay-Russell, {Michele T} and Mandrell, {Robert E.} and Jean Yuan and Anna Bates and Rosa Manalac and Janet Mohle-Boetani and Akiko Kimura and Janice Lidgard and Miller, {William G.}",
year = "2013",
month = "1",
doi = "10.1128/JCM.01845-12",
language = "English (US)",
volume = "51",
pages = "195--201",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California

AU - Jay-Russell, Michele T

AU - Mandrell, Robert E.

AU - Yuan, Jean

AU - Bates, Anna

AU - Manalac, Rosa

AU - Mohle-Boetani, Janet

AU - Kimura, Akiko

AU - Lidgard, Janice

AU - Miller, William G.

PY - 2013/1

Y1 - 2013/1

N2 - We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7%) bovine fecal, 12 of 20 (60%) flush alley water, and 1 of 20 (5%) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6%) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.

AB - We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7%) bovine fecal, 12 of 20 (60%) flush alley water, and 1 of 20 (5%) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6%) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.

UR - http://www.scopus.com/inward/record.url?scp=84871689690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871689690&partnerID=8YFLogxK

U2 - 10.1128/JCM.01845-12

DO - 10.1128/JCM.01845-12

M3 - Article

VL - 51

SP - 195

EP - 201

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -