Abstract
Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 361-365 |
| Number of pages | 5 |
| Journal | Acta Histochemica et Cytochemica |
| Volume | 35 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2002 |
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Keywords
- Antisense
- Chicken
- Electroporation
- Morpholino
- Tenascin-C
ASJC Scopus subject areas
- Biochemistry
- Neuroscience(all)
- Endocrinology
- Anatomy
- Cell Biology
Cite this
Using antisense morpholino oligos to knockdown gene expression in the chicken embryo. / Tucker, Richard P.
In: Acta Histochemica et Cytochemica, Vol. 35, No. 5, 2002, p. 361-365.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Using antisense morpholino oligos to knockdown gene expression in the chicken embryo
AU - Tucker, Richard P
PY - 2002
Y1 - 2002
N2 - Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.
AB - Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.
KW - Antisense
KW - Chicken
KW - Electroporation
KW - Morpholino
KW - Tenascin-C
UR - http://www.scopus.com/inward/record.url?scp=0036909781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036909781&partnerID=8YFLogxK
U2 - 10.1267/ahc.35.361
DO - 10.1267/ahc.35.361
M3 - Article
AN - SCOPUS:0036909781
VL - 35
SP - 361
EP - 365
JO - Acta Histochemica et Cytochemica
JF - Acta Histochemica et Cytochemica
SN - 0044-5991
IS - 5
ER -