Using antisense morpholino oligos to knockdown gene expression in the chicken embryo

Richard P Tucker

doi:10.1267/ahc.35.361

Using antisense morpholino oligos to knockdown gene expression in the chicken embryo

Richard P Tucker

Cell Biology and Human Anatomy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.

Original language	English (US)
Pages (from-to)	361-365
Number of pages	5
Journal	Acta Histochemica et Cytochemica
Volume	35
Issue number	5
DOIs	https://doi.org/10.1267/ahc.35.361
State	Published - 2002

Keywords

Antisense
Chicken
Electroporation
Morpholino
Tenascin-C

ASJC Scopus subject areas

Biochemistry
Neuroscience(all)
Endocrinology
Anatomy
Cell Biology

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10.1267/ahc.35.361

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abstract = "Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.",

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AB - Morpholinos are synthetic DNA analogs that are not degraded by nucleases. A technique has been developed for the microinjection and square-pulse electroporation of antisense morpholinos into the precursors of neural crest cells in the chicken embryo in ovo. This paper reviews the methods used for the introduction of morpholinos into the chicken embryo as well as how to analyze their effects on gene expression and morphogenesis.

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KW - Electroporation

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KW - Tenascin-C

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Using antisense morpholino oligos to knockdown gene expression in the chicken embryo

Abstract

Keywords

ASJC Scopus subject areas

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