Uses of thrombin in ocular surgery: Effect on the corneal endothelium

Mark J Mannis, E. Sweet, M. B. Landers, R. A. Lewis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Thrombin is a hemostatic factor that induces platelet aggregation and catalyzes the conversion of fibrinogen to fibrin. The potential for its increasing application to a variety of anterior and posterior segment surgery led us to investigate the in vitro effect of thrombin on the corneal endothelium in a sheep model. We examined freshly excised sheep corneas stained with alizarin red and trypan blue after exposure to two different concentrations of thrombin for four hours. The structure of the corneal endothelium appeared to be intact even after prolonged exposure to thrombin at concentrations of 100 and 1000 U/mL. Thrombin appears to be nontoxic to the corneal endothelium in this experimental model.

Original languageEnglish (US)
Pages (from-to)251-253
Number of pages3
JournalArchives of Ophthalmology
Volume106
Issue number2
StatePublished - 1988

Fingerprint

Corneal Endothelium
Thrombin
Sheep
Trypan Blue
Hemostatics
Fibrin
Platelet Aggregation
Cornea
Fibrinogen
Theoretical Models

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Mannis, M. J., Sweet, E., Landers, M. B., & Lewis, R. A. (1988). Uses of thrombin in ocular surgery: Effect on the corneal endothelium. Archives of Ophthalmology, 106(2), 251-253.

Uses of thrombin in ocular surgery : Effect on the corneal endothelium. / Mannis, Mark J; Sweet, E.; Landers, M. B.; Lewis, R. A.

In: Archives of Ophthalmology, Vol. 106, No. 2, 1988, p. 251-253.

Research output: Contribution to journalArticle

Mannis, MJ, Sweet, E, Landers, MB & Lewis, RA 1988, 'Uses of thrombin in ocular surgery: Effect on the corneal endothelium', Archives of Ophthalmology, vol. 106, no. 2, pp. 251-253.
Mannis, Mark J ; Sweet, E. ; Landers, M. B. ; Lewis, R. A. / Uses of thrombin in ocular surgery : Effect on the corneal endothelium. In: Archives of Ophthalmology. 1988 ; Vol. 106, No. 2. pp. 251-253.
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