Abstract
Thrombin is a hemostatic factor that induces platelet aggregation and catalyzes the conversion of fibrinogen to fibrin. The potential for its increasing application to a variety of anterior and posterior segment surgery led us to investigate the in vitro effect of thrombin on the corneal endothelium in a sheep model. We examined freshly excised sheep corneas stained with alizarin red and trypan blue after exposure to two different concentrations of thrombin for four hours. The structure of the corneal endothelium appeared to be intact even after prolonged exposure to thrombin at concentrations of 100 and 1000 U/mL. Thrombin appears to be nontoxic to the corneal endothelium in this experimental model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 251-253 |
| Number of pages | 3 |
| Journal | Archives of Ophthalmology |
| Volume | 106 |
| Issue number | 2 |
| State | Published - 1988 |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Uses of thrombin in ocular surgery : Effect on the corneal endothelium. / Mannis, Mark J; Sweet, E.; Landers, M. B.; Lewis, R. A.
In: Archives of Ophthalmology, Vol. 106, No. 2, 1988, p. 251-253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Uses of thrombin in ocular surgery
T2 - Effect on the corneal endothelium
AU - Mannis, Mark J
AU - Sweet, E.
AU - Landers, M. B.
AU - Lewis, R. A.
PY - 1988
Y1 - 1988
N2 - Thrombin is a hemostatic factor that induces platelet aggregation and catalyzes the conversion of fibrinogen to fibrin. The potential for its increasing application to a variety of anterior and posterior segment surgery led us to investigate the in vitro effect of thrombin on the corneal endothelium in a sheep model. We examined freshly excised sheep corneas stained with alizarin red and trypan blue after exposure to two different concentrations of thrombin for four hours. The structure of the corneal endothelium appeared to be intact even after prolonged exposure to thrombin at concentrations of 100 and 1000 U/mL. Thrombin appears to be nontoxic to the corneal endothelium in this experimental model.
AB - Thrombin is a hemostatic factor that induces platelet aggregation and catalyzes the conversion of fibrinogen to fibrin. The potential for its increasing application to a variety of anterior and posterior segment surgery led us to investigate the in vitro effect of thrombin on the corneal endothelium in a sheep model. We examined freshly excised sheep corneas stained with alizarin red and trypan blue after exposure to two different concentrations of thrombin for four hours. The structure of the corneal endothelium appeared to be intact even after prolonged exposure to thrombin at concentrations of 100 and 1000 U/mL. Thrombin appears to be nontoxic to the corneal endothelium in this experimental model.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023857517&partnerID=8YFLogxK
M3 - Article
C2 - 3341983
AN - SCOPUS:0023857517
VL - 106
SP - 251
EP - 253
JO - JAMA Ophthalmology
JF - JAMA Ophthalmology
SN - 2168-6165
IS - 2
ER -