Abstract
The incidence of pulmonary fibrosis increases with age. Studies from our group have implicated circulating progenitor cells, termed fibrocytes, in lung fibrosis. In this study, we investigate whether the preceding determinants of inflammation and fibrosis were augmented with aging. We compared responses to intratracheal bleomycin in senescence-accelerated prone mice (SAMP), with responses in age-matched control senescence-accelerated resistant mice (SAMR). SAMP mice demonstrated an exaggerated inflammatory response as evidenced by lung histology. Bleomycin-induced fibrosis was significantly higher in SAMP mice compared with SAMR controls. Consistent with fibrotic changes in the lung, SAMP mice expressed higher levels of transforming growth factor-β1 in the lung. Furthermore, SAMP mice showed higher numbers of fibrocytes and higher levels of stromal cell-derived factor-1 in the peripheral blood. This study provides the novel observation that apart from increases in inflammatory and fibrotic factors in response to injury, the increased mobilization of fibrocytes may be involved in age-related susceptibility to lung fibrosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 731-739 |
| Number of pages | 9 |
| Journal | Journals of Gerontology - Series A Biological Sciences and Medical Sciences |
| Volume | 64 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2009 |
| Externally published | Yes |
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Keywords
- Bone marrow
- Lung
- Mice
- Senescence
ASJC Scopus subject areas
- Aging
- Geriatrics and Gerontology
Cite this
Use of senescence-accelerated mouse model in bleomycin-induced lung injury suggests that bone marrow-derived cells can alter the outcome of lung injury in aged mice. / Xu, Jianguo; Gonzalez, Edilson T.; Iyer, Smita; Mac, Valerie; Mora, Ana L.; Sutliff, Roy L.; Reed, Alana; Brigham, Kenneth L.; Kelly, Patricia; Rojas, Mauricio.
In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 64, No. 7, 01.07.2009, p. 731-739.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of senescence-accelerated mouse model in bleomycin-induced lung injury suggests that bone marrow-derived cells can alter the outcome of lung injury in aged mice
AU - Xu, Jianguo
AU - Gonzalez, Edilson T.
AU - Iyer, Smita
AU - Mac, Valerie
AU - Mora, Ana L.
AU - Sutliff, Roy L.
AU - Reed, Alana
AU - Brigham, Kenneth L.
AU - Kelly, Patricia
AU - Rojas, Mauricio
PY - 2009/7/1
Y1 - 2009/7/1
N2 - The incidence of pulmonary fibrosis increases with age. Studies from our group have implicated circulating progenitor cells, termed fibrocytes, in lung fibrosis. In this study, we investigate whether the preceding determinants of inflammation and fibrosis were augmented with aging. We compared responses to intratracheal bleomycin in senescence-accelerated prone mice (SAMP), with responses in age-matched control senescence-accelerated resistant mice (SAMR). SAMP mice demonstrated an exaggerated inflammatory response as evidenced by lung histology. Bleomycin-induced fibrosis was significantly higher in SAMP mice compared with SAMR controls. Consistent with fibrotic changes in the lung, SAMP mice expressed higher levels of transforming growth factor-β1 in the lung. Furthermore, SAMP mice showed higher numbers of fibrocytes and higher levels of stromal cell-derived factor-1 in the peripheral blood. This study provides the novel observation that apart from increases in inflammatory and fibrotic factors in response to injury, the increased mobilization of fibrocytes may be involved in age-related susceptibility to lung fibrosis.
AB - The incidence of pulmonary fibrosis increases with age. Studies from our group have implicated circulating progenitor cells, termed fibrocytes, in lung fibrosis. In this study, we investigate whether the preceding determinants of inflammation and fibrosis were augmented with aging. We compared responses to intratracheal bleomycin in senescence-accelerated prone mice (SAMP), with responses in age-matched control senescence-accelerated resistant mice (SAMR). SAMP mice demonstrated an exaggerated inflammatory response as evidenced by lung histology. Bleomycin-induced fibrosis was significantly higher in SAMP mice compared with SAMR controls. Consistent with fibrotic changes in the lung, SAMP mice expressed higher levels of transforming growth factor-β1 in the lung. Furthermore, SAMP mice showed higher numbers of fibrocytes and higher levels of stromal cell-derived factor-1 in the peripheral blood. This study provides the novel observation that apart from increases in inflammatory and fibrotic factors in response to injury, the increased mobilization of fibrocytes may be involved in age-related susceptibility to lung fibrosis.
KW - Bone marrow
KW - Lung
KW - Mice
KW - Senescence
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UR - http://www.scopus.com/inward/citedby.url?scp=67649651896&partnerID=8YFLogxK
U2 - 10.1093/gerona/glp040
DO - 10.1093/gerona/glp040
M3 - Article
VL - 64
SP - 731
EP - 739
JO - The journals of gerontology. Series A, Biological sciences and medical sciences
JF - The journals of gerontology. Series A, Biological sciences and medical sciences
SN - 1079-5006
IS - 7
ER -